Enzyme-assisted three phase partitioning: A novel approach for extraction of turmeric oleoresin

Nilesh N. Kurmudle; Sandip B. Bankar; Ishwar B. Bajaj; Mahesh V. Bule; Rekha S. Singhal

doi:10.1016/j.procbio.2010.09.010

Enzyme-assisted three phase partitioning: A novel approach for extraction of turmeric oleoresin

Nilesh N. Kurmudle, Sandip B. Bankar, Ishwar B. Bajaj, Mahesh V. Bule, Rekha S. Singhal

Not published at Aalto University

Research output: Contribution to journal › Article › Scientific › peer-review

32 Citations (Scopus)

Abstract

Three phase partitioning, a technique which has been explored for protein separation was evaluated for extraction of turmeric oleoresin. The process consists of simultaneous addition of t-butanol and ammonium sulphate to the aqueous slurry of turmeric powder. The extraction of oleoresin was optimized with respect to the concentration of ammonium sulphate loading and the ratio of t-butanol to slurry. Pretreatment of the slurries with a commercial enzyme preparation of α-amylase and/or glucoamylase followed by three phase partitioning was carried out. The extraction time with this technique is lower as compared to conventional acetone extraction.

Original language	English
Pages (from-to)	423-426
Number of pages	4
Journal	Process Biochemistry
Volume	46
Issue number	1
DOIs	https://doi.org/10.1016/j.procbio.2010.09.010
Publication status	Published - Jan 2011
MoE publication type	A1 Journal article-refereed

Keywords

α-Amylase
Enzyme assisted three phase partitioning
Extraction
Glucoamylase
Turmeric oleoresin

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10.1016/j.procbio.2010.09.010

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title = "Enzyme-assisted three phase partitioning: A novel approach for extraction of turmeric oleoresin",

abstract = "Three phase partitioning, a technique which has been explored for protein separation was evaluated for extraction of turmeric oleoresin. The process consists of simultaneous addition of t-butanol and ammonium sulphate to the aqueous slurry of turmeric powder. The extraction of oleoresin was optimized with respect to the concentration of ammonium sulphate loading and the ratio of t-butanol to slurry. Pretreatment of the slurries with a commercial enzyme preparation of α-amylase and/or glucoamylase followed by three phase partitioning was carried out. The extraction time with this technique is lower as compared to conventional acetone extraction.",

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AU - Bule, Mahesh V.

AU - Singhal, Rekha S.

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