Enzyme-assisted three phase partitioning: A novel approach for extraction of turmeric oleoresin

Nilesh N. Kurmudle, Sandip B. Bankar, Ishwar B. Bajaj, Mahesh V. Bule, Rekha S. Singhal

Research output: Contribution to journalArticleScientificpeer-review

32 Citations (Scopus)

Abstract

Three phase partitioning, a technique which has been explored for protein separation was evaluated for extraction of turmeric oleoresin. The process consists of simultaneous addition of t-butanol and ammonium sulphate to the aqueous slurry of turmeric powder. The extraction of oleoresin was optimized with respect to the concentration of ammonium sulphate loading and the ratio of t-butanol to slurry. Pretreatment of the slurries with a commercial enzyme preparation of α-amylase and/or glucoamylase followed by three phase partitioning was carried out. The extraction time with this technique is lower as compared to conventional acetone extraction.

Original languageEnglish
Pages (from-to)423-426
Number of pages4
JournalProcess Biochemistry
Volume46
Issue number1
DOIs
Publication statusPublished - Jan 2011
MoE publication typeA1 Journal article-refereed

Keywords

  • α-Amylase
  • Enzyme assisted three phase partitioning
  • Extraction
  • Glucoamylase
  • Turmeric oleoresin

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