Enzymatic production of 4-O-methyl d-glucaric acid from hardwood xylan

Thu V. Vuong, Emma R. Master*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

5 Citations (Scopus)
31 Downloads (Pure)

Abstract

Background: Dicarboxylic acids offer several applications in detergent builder and biopolymer fields. One of these acids, 4-O-methyl d-glucaric acid, could potentially be produced from glucuronoxylans, which are a comparatively underused fraction of wood and agricultural biorefineries. Results: Accordingly, an enzymatic pathway was developed that combines AxyAgu115A, a GH115 α-glucuronidase from Amphibacillus xylanus, and GOOX, an AA7 gluco-oligosaccharide oxidase from Sarocladium strictum, to produce this bio-based chemical from glucuronoxylan. AxyAgu115A was able to release almost all 4-O-methyl d-glucuronic acid from glucuronoxylan while a GOOX variant, GOOX-Y300A, could convert 4-O-methyl d-glucuronic acid to the corresponding glucaric acid at a yield of 62%. Both enzymes worked effectively at alkaline conditions that increase xylan solubility. Given the sensitivity of AxyAgu115A to hydrogen peroxide and optimal performance of GOOX-Y300A at substrate concentrations above 20 mM, the two-step enzyme pathway was demonstrated as a sequential, one-pot reaction. Additionally, the resulting xylan was easily recovered from the one-pot reaction, and it was enzymatically hydrolysable. Conclusions: The pathway in this study requires only two enzymes while avoiding a supplementation of costly cofactors. This cell-free approach provides a new strategy to make use of the underutilized hemicellulose stream from wood and agricultural biorefineries.

Original languageEnglish
Article number51
Number of pages10
JournalBiotechnology for Biofuels
Volume13
Issue number1
DOIs
Publication statusPublished - 13 Mar 2020
MoE publication typeA1 Journal article-refereed

Keywords

  • 4-O-Methyl d-glucaric acid
  • AA7
  • Biorefinery
  • GH115
  • Hemicellulose
  • Xylan

Fingerprint

Dive into the research topics of 'Enzymatic production of 4-O-methyl d-glucaric acid from hardwood xylan'. Together they form a unique fingerprint.

Cite this