Enzymatic hydrolysis of microcrystalline cellulose and pretreated wheat straw: A detailed comparison using convenient kinetic analysis

Mareike Monschein, Christoph Reisinger, Bernd Nidetzky*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

18 Citations (Scopus)

Abstract

Marked slow-down of soluble sugar production at low degree of substrate conversion limits the space-time yield of enzymatic hydrolysis of ligno-cellulosic materials. A simple set of kinetic descriptors was developed to compare reducing sugar release from pure crystalline cellulose (Avicel) and pretreated wheat straw by Trichoderma reesei cellulase at 50°C. The focus was on the rate-retarding effect of maximum hydrolysis rate at reaction start (rmax), limiting hydrolysis rate (rlim) at extended reaction time (24h), and substrate conversion, marking the transition between the rmax and rlim kinetic regimes (Ctrans). At apparent saturation of substrate (12.2g cellulose/L) with enzyme, rmax for pretreated wheat straw (~9.6g/L/h) surpassed that for Avicel by about 1.7-fold whereas their rlim were almost identical (~0.15g/L/h). Ctrans roughly doubled as enzyme/substrate loading was increased from 3.8 to 75FPU/g, suggesting Ctrans to be a complex manifestation of cellulase-cellulose interaction, not an intrinsic substrate property. A low-temperature adsorption step preceding hydrolysis at 50°C resulted in enhanced cellulase binding at reaction start without increasing rmax. Ctrans was higher for pretreated wheat straw (~30%) than for Avicel (~20%) under these conditions.

Original languageEnglish
Pages (from-to)679-687
Number of pages9
JournalBioresource Technology
Volume128
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

Keywords

  • Adsorption
  • Enzymatic hydrolysis
  • Hydrolysis rate
  • Lignocellulose
  • Microcrystalline cellulose

Fingerprint Dive into the research topics of 'Enzymatic hydrolysis of microcrystalline cellulose and pretreated wheat straw: A detailed comparison using convenient kinetic analysis'. Together they form a unique fingerprint.

  • Cite this