Enzymatic hydrolysis of biomimetic bacterial cellulose–hemicellulose composites

Paavo A. Penttilä*, Tomoya Imai, Jarl Hemming, Stefan Willför, Junji Sugiyama

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

13 Citations (Scopus)

Abstract

The production of biofuels and other chemicals from lignocellulosic biomass is limited by the inefficiency of enzymatic hydrolysis. Here a biomimetic composite material consisting of bacterial cellulose and wood-based hemicelluloses was used to study the effects of hemicelluloses on the enzymatic hydrolysis with a commercial cellulase mixture. Bacterial cellulose synthesized in the presence of hemicelluloses, especially xylan, was found to be more susceptible to enzymatic hydrolysis than hemicellulose-free bacterial cellulose. The reason for the easier hydrolysis could be related to the nanoscale structure of the substrate, particularly the packing of cellulose microfibrils into ribbons or bundles. In addition, small-angle X-ray scattering was used to show that the average nanoscale morphology of bacterial cellulose remained unchanged during the enzymatic hydrolysis. The reported easier enzymatic hydrolysis of bacterial cellulose produced in the presence of wood-based xylan offers new insights to overcome biomass recalcitrance through genetic engineering.

Original languageEnglish
Pages (from-to)95-102
Number of pages8
JournalCarbohydrate Polymers
Volume190
DOIs
Publication statusPublished - 15 Jun 2018
MoE publication typeA1 Journal article-refereed

Keywords

  • Bacterial cellulose
  • Enzymatic hydrolysis
  • Hemicellulose
  • Small-angle X-ray scattering

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