Enhancing the flux of D-glucose to the pentose phosphate pathway in Saccharomyces cerevisiae for the production of D-ribose and ribitol

Research output: Contribution to journalArticle

Researchers

  • Mervi H. Toivari
  • Hannu Maaheimo
  • Merja Penttila
  • Laura Ruohonen

Research units

  • VTT Technical Research Centre of Finland

Abstract

Phosphoglucose isomerase-deficient (pgi1) strains of Saccharomyces cerevisiae were studied for the production of D-ribose and ribitol from D-glucose via the intermediates of the pentose phosphate pathway. Overexpression of the genes coding for NAD(+)-specific glutamate dehydrogenase (GDH2) of S. cerevisiae or NADPH-utilising glyceraldehyde-3-phosphate dehydrogenase (gapB) of Bacillus subtilis enabled growth of the pgi1 mutant strains on D-glucose. Overexpression of the gene encoding sugar phosphate phosphatase (DOG1) of S. cerevisiae was needed for the production of D-ribose and ribitol; however, it reduced the growth of the pgi1 strains expressing GDH2 or gapB in the presence of higher D-glucose concentrations. The CEN.PK2-1D laboratory strain expressing both gapB and DOG1 produced approximately 0.4 g l(-1) of D-ribose and ribitol when grown on 20 g l(-1) (w/v) D-fructose with 4 g l(-1) (w/v) D-glucose. Nuclear magnetic resonance measurements of the cells grown with (13)C-labelled D-glucose showed that about 60% of the D-ribose produced was derived from D-glucose. Strains deficient in both phosphoglucose isomerase and transketolase activities, and expressing DOG1 and GDH2 tolerated only low D-glucose concentrations (a parts per thousand currency sign2 g l(-1) (w/v)), but produced 1 g l(-1) (w/v) D-ribose and ribitol when grown on 20 g l(-1) (w/v) D-fructose with 2 g l(-1) (w/v) D-glucose.

Details

Original languageEnglish
Pages (from-to)731-739
Number of pages9
JournalApplied Microbiology and Biotechnology
Volume85
Issue number3
Publication statusPublished - Jan 2010
MoE publication typeA1 Journal article-refereed

    Research areas

  • Sugar alcohols, Pentose sugars, Saccharomyces cerevisiae, Pentose phosphate pathway, D-ribose, Ribitol, NMR, KLUYVEROMYCES-LACTIS, BACILLUS-SUBTILIS, ESCHERICHIA-COLI, ISOMERASE MUTANT, AMINO-ACIDS, STRAINS, YEAST, IDENTIFICATION, METABOLISM, VECTORS

ID: 9229240