Abstract
Phosphoglucose isomerase-deficient (pgi1) strains of Saccharomyces cerevisiae were studied for the production of D-ribose and ribitol from D-glucose via the intermediates of the pentose phosphate pathway. Overexpression of the genes coding for NAD(+)-specific glutamate dehydrogenase (GDH2) of S. cerevisiae or NADPH-utilising glyceraldehyde-3-phosphate dehydrogenase (gapB) of Bacillus subtilis enabled growth of the pgi1 mutant strains on D-glucose. Overexpression of the gene encoding sugar phosphate phosphatase (DOG1) of S. cerevisiae was needed for the production of D-ribose and ribitol; however, it reduced the growth of the pgi1 strains expressing GDH2 or gapB in the presence of higher D-glucose concentrations. The CEN.PK2-1D laboratory strain expressing both gapB and DOG1 produced approximately 0.4 g l(-1) of D-ribose and ribitol when grown on 20 g l(-1) (w/v) D-fructose with 4 g l(-1) (w/v) D-glucose. Nuclear magnetic resonance measurements of the cells grown with (13)C-labelled D-glucose showed that about 60% of the D-ribose produced was derived from D-glucose. Strains deficient in both phosphoglucose isomerase and transketolase activities, and expressing DOG1 and GDH2 tolerated only low D-glucose concentrations (a parts per thousand currency sign2 g l(-1) (w/v)), but produced 1 g l(-1) (w/v) D-ribose and ribitol when grown on 20 g l(-1) (w/v) D-fructose with 2 g l(-1) (w/v) D-glucose.
Original language | English |
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Pages (from-to) | 731-739 |
Number of pages | 9 |
Journal | Applied Microbiology and Biotechnology |
Volume | 85 |
Issue number | 3 |
DOIs | |
Publication status | Published - Jan 2010 |
MoE publication type | A1 Journal article-refereed |
Keywords
- Sugar alcohols
- Pentose sugars
- Saccharomyces cerevisiae
- Pentose phosphate pathway
- D-ribose
- Ribitol
- NMR
- KLUYVEROMYCES-LACTIS
- BACILLUS-SUBTILIS
- ESCHERICHIA-COLI
- ISOMERASE MUTANT
- AMINO-ACIDS
- STRAINS
- YEAST
- IDENTIFICATION
- METABOLISM
- VECTORS