Enhanced bone mineralization using hydroxyapatite-based ceramic bone substitute incorporating Withania somnifera extracts

Irfan Qayoom, Arun Kumar Teotia, Megha Meena, Prerna Singh, Ankita Mishra, Sneha Singh, Ashok Kumar

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Withania somnifera (ashwagandha) is used in Indian traditional medicine for its various health benefits. Withaferin-A, a steroidal lactone present in this herb, has shown proteosomal inhibition-based enhancement of bone mineralization. In the present work, chitosan microparticles blended with total methanolic root extract of W. somnifera were incorporated as a porogen in calcium phosphate-based hydroxyapatite bone filler. The controlled release of bioactive molecules enabled enhanced proliferation and differentiation of pre-osteoblasts. Microparticle percentages were optimized to have a minimum effect on the setting time, mechanical strength and degradability of hydroxyapatite bone filler. In vitro cell adhesion, proliferation and differentiation were evaluated to determine the biocompatibility of the composites. On the basis of the desirable results obtained, we provide a preliminary rationale for the use of methanolic extract-blended chitosan microparticle-impregnated calcium phosphate filler for enhanced bone regeneration.
Original languageEnglish
Article number055015
Number of pages15
JournalBIOMEDICAL MATERIALS
Volume15
Issue number5
DOIs
Publication statusPublished - 16 Jul 2020
MoE publication typeA1 Journal article-refereed

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