Engineered protein cages for selective heparin encapsulation

Salla Välimäki; Qing Liu; Lise Schoonen; Daan F. M. Vervoort; Nonappa Nonappa; Veikko Linko; Roeland J. M. Nolte; Jan C. M. van Hest; Mauri Kostiainen

doi:10.1039/D0TB02541K

Engineered protein cages for selective heparin encapsulation

Salla Välimäki, Qing Liu, Lise Schoonen, Daan F. M. Vervoort, Nonappa Nonappa, Veikko Linko, Roeland J. M. Nolte, Jan C. M. van Hest, Mauri Kostiainen

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Abstract

A heparin-specific binding peptide was conjugated to a cowpea chlorotic mottle virus (CCMV) capsid protein, which was subsequently allowed to encapsulate heparin and form capsid-like protein cages. The encapsulation is specific and the capsid-heparin assemblies display negligible hemolytic activity, indicating proper blood compatibility and promising possibilities for heparin antidote applications.

Original language	English
Pages (from-to)	1272-1276
Number of pages	5
Journal	Journal of Materials Chemistry B
Volume	9
Issue number	5
DOIs	https://doi.org/10.1039/D0TB02541K
Publication status	Published - 7 Feb 2021
MoE publication type	A1 Journal article-refereed

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SYNVIRO / Kostiainen
Kostiainen, M., Ahmed, A., Anaya Plaza, E., Liu, Q., Julin, S., Välimäki, S. & Korpi, A.
01/09/2017 → 31/08/2021
Project: Academy of Finland: Other research funding
Kostiainen Mauri AT-kulut
Kostiainen, M., Korpi, A., Damania, A., Liljeström, V. & Ora, A.
01/09/2016 → 31/08/2018
Project: Academy of Finland: Other research funding

Bioeconomy Research Infrastructure
Jukka Seppälä (Manager)
School of Chemical Engineering
Facility/equipment: Facility
OtaNano
Anna Rissanen (Manager)
Aalto University
Facility/equipment: Facility
OtaNano - Nanomicroscopy Center
Jani Seitsonen (Manager)
OtaNano
Facility/equipment: Facility

Cite this

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title = "Engineered protein cages for selective heparin encapsulation",

abstract = "A heparin-specific binding peptide was conjugated to a cowpea chlorotic mottle virus (CCMV) capsid protein, which was subsequently allowed to encapsulate heparin and form capsid-like protein cages. The encapsulation is specific and the capsid-heparin assemblies display negligible hemolytic activity, indicating proper blood compatibility and promising possibilities for heparin antidote applications.",

author = "Salla V{\"a}lim{\"a}ki and Qing Liu and Lise Schoonen and {F. M. Vervoort}, Daan and Nonappa Nonappa and Veikko Linko and Nolte, {Roeland J. M.} and {C. M. van Hest}, Jan and Mauri Kostiainen",

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AU - Välimäki, Salla

AU - Liu, Qing

AU - Schoonen, Lise

AU - F. M. Vervoort, Daan

AU - Nonappa, Nonappa

AU - Linko, Veikko

AU - Nolte, Roeland J. M.

AU - C. M. van Hest, Jan

AU - Kostiainen, Mauri

PY - 2021/2/7

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AB - A heparin-specific binding peptide was conjugated to a cowpea chlorotic mottle virus (CCMV) capsid protein, which was subsequently allowed to encapsulate heparin and form capsid-like protein cages. The encapsulation is specific and the capsid-heparin assemblies display negligible hemolytic activity, indicating proper blood compatibility and promising possibilities for heparin antidote applications.

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DO - 10.1039/D0TB02541K

M3 - Article

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JF - JOURNAL OF MATERIALS CHEMISTRY. B

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IS - 5

ER -

Engineered protein cages for selective heparin encapsulation

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SYNVIRO / Kostiainen

Kostiainen Mauri AT-kulut

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Bioeconomy Research Infrastructure

OtaNano

OtaNano - Nanomicroscopy Center

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