Projects per year
Abstract
Protein and peptide cages are nanoscale containers, which are of particular interest in nanoscience due to their well-defined dimensions and enclosed central cavities that can be filled with material that is protected from the outside environment. Ferritin is a typical example of protein cage, formed by 24 polypeptide chains that self-assemble into a hollow, roughly spherical protein cage with external and internal diameters of approximately 12 nm and 8 nm, respectively. The interior cavity of ferritin provides a unique reaction vessel to carry out reactions separated from the exterior environment. In nature, the cavity is utilized for sequestration and biomineralization to render iron inert and safe by shielding from the external environment. Materials scientists have been inspired by this system and exploited a range of ferritin superfamily proteins as supramolecular templates to encapsulate cargoes ranging from cancer drugs to therapeutic proteins. Interesting possibilities arise if such containers can themselves be arranged into even higher-order structures such as crystalline arrays. Here, we describe how crystalline arrays of negatively charged ferritin protein cages can be built by taking advantage of electrostatic interactions with cationic gold nanoparticles.
Original language | English |
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Title of host publication | Polypeptide materials : methods and protocols |
Editors | Maxim G. Ryadnov |
Publisher | Springer |
Pages | 123-133 |
Number of pages | 11 |
Volume | 2208 |
ISBN (Electronic) | 978-1-0716-0928-6 |
ISBN (Print) | 978-1-0716-0927-9 |
DOIs | |
Publication status | Published - 1 Jan 2021 |
MoE publication type | A3 Book section, Chapters in research books |
Publication series
Name | Methods in molecular biology |
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Publisher | Humana Press |
Volume | 2208 |
ISSN (Print) | 1064-3745 |
ISSN (Electronic) | 1940-6029 |
Keywords
- Nanocontainers
- Nanocrystals
- Protein cage
- Protein design
- Protein engineering
Fingerprint
Dive into the research topics of 'Electrostatic Self-Assembly of Protein Cage Arrays'. Together they form a unique fingerprint.Projects
- 2 Finished
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SYNVIRO: Synthetic Virology Toolbox for the Encapsulation of Therapeutic Polyelectrolytes (SYNVIRO)
Kostiainen, M. (Principal investigator)
01/09/2017 → 31/08/2021
Project: Academy of Finland: Other research funding
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Protein Cage Directed Self-Assembly of Nanomaterials
Kostiainen, M. (Principal investigator)
01/09/2016 → 31/08/2018
Project: Academy of Finland: Other research funding
Equipment
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OtaNano - Nanomicroscopy Center
Seitsonen, J. (Manager) & Rissanen, A. (Other)
OtaNanoFacility/equipment: Facility