Electron cryotomography of measles virus reveals how matrix protein coats the ribonucleocapsid within intact virions

Research output: Contribution to journalArticleScientificpeer-review

Researchers

  • Lassi Liljeroos
  • Juha T. Huiskonen
  • Ari Ora

  • Petri Susi
  • Sarah J. Butcher

Research units

  • University of Helsinki
  • University of Oxford
  • University of Turku

Abstract

Measles virus is a highly infectious, enveloped, pleomorphic virus. We combined electron cryotomography with subvolume averaging and immunosorbent electron microscopy to characterize the 3D ultrastructure of the virion. We show that the matrix protein forms helices coating the helical ribonucleocapsid rather than coating the inner leaflet of the membrane, as previously thought. The ribonucleocapsid is folded into tight bundles through matrix-matrix interactions. The implications for virus assembly are that the matrix already tightly interacts with the ribonucleocapsid in the cytoplasm, providing a structural basis for the previously observed regulation of RNA transcription by the matrix protein. Next, the matrix-covered ribonucleocapsids are transported to the plasma membrane, where the matrix interacts with the envelope glycoproteins during budding. These results are relevant to the nucleocapsid organization and budding of other paramyxoviruses, where isolated matrix has been observed to form helices.

Details

Original languageEnglish
Pages (from-to)18085-18090
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number44
Publication statusPublished - 1 Nov 2011
MoE publication typeA1 Journal article-refereed

    Research areas

  • Image reconstruction, Subtomogram averaging

ID: 31968880