Electrochemical detection of hydrogen peroxide on platinum-containing tetrahedral amorphous carbon sensors and evaluation of their biofouling properties

Noora Tujunen, Emilia Kaivosoja, Vera Protopopova, Juan José Valle-Delgado, Monika Österberg, Jari Koskinen, Tomi Laurila*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)
314 Downloads (Pure)

Abstract

Hydrogen peroxide is the product of various enzymatic reactions, and is thus typically utilized as the analyte in biosensors. However, its detection with conventional materials, such as noble metals or glassy carbon, is often hindered by slow kinetics and biofouling of the electrode. In this study electrochemical properties and suitability to peroxide detection as well as ability to resist biofouling of Pt-doped ta-C samples were evaluated. Pure ta-C and pure Pt were used as references. According to the results presented here it is proposed that combining ta-C with Pt results in good electrocatalytic activity towards H2O2 oxidation with better tolerance towards aqueous environment mimicking physiological conditions compared to pure Pt. In biofouling experiments, however, both the hybrid material and Pt were almost completely, blocked after immersion in protein-containing solutions and did not produce any peaks for ferrocenemethanol oxidation or reduction. On the contrary, it was still possible to obtain clear peaks for H2O2 oxidation with them after similar treatment Moreover, quartz crystal microbalance experiment showed less protein adsorption on the hybrid sample compared to Pt which is also supported by the electrochemical biofouling experiments for H2O2 detection. (C) 2015 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)70-78
Number of pages9
JournalMATERIALS SCIENCE AND ENGINEERING C: MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume55
DOIs
Publication statusPublished - 1 Oct 2015
MoE publication typeA1 Journal article-refereed

Keywords

  • Tetrahedral amorphous carbon
  • Platinum
  • Hydrogen peroxide
  • Sensor
  • Biofouling

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