Elastic and pH-Responsive Hybrid Interfaces Created with Engineered Resilin and Nanocellulose

Wenwen Fang; Arja Paananen; Marika Vitikainen; Salla Koskela; Ann Westerholm-Parvinen; Jussi J. Joensuu; Christopher P. Landowski; Merja Penttilä; Markus Linder; Päivi Laaksonen

doi:10.1021/acs.biomac.7b00294

Elastic and pH-Responsive Hybrid Interfaces Created with Engineered Resilin and Nanocellulose

Wenwen Fang, Arja Paananen, Marika Vitikainen, Salla Koskela, Ann Westerholm-Parvinen, Jussi J. Joensuu, Christopher P. Landowski, Merja Penttilä, Markus Linder, Päivi Laaksonen

VTT Technical Research Centre of Finland

Research output: Contribution to journal › Article › Scientific › peer-review

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Abstract

We investigated how a genetically engineered resilin fusion protein modifies cellulose surfaces. We characterized the pH-responsive behavior of a resilin-like polypeptide (RLP) having terminal cellulose binding modules (CBM) and showed its binding to cellulose nanofibrils (CNF). Characterization of the resilin fusion protein at different pHs revealed substantial conformational changes of the protein, which were observed as swelling and contraction of the protein layer bound to the nanocellulose surface. In addition, we showed that employment of the modified resilin in cellulose hydrogel and nanopaper increased their modulus of stiffness through a cross-linking effect.

Original language	English
Pages (from-to)	1866-1873
Number of pages	8
Journal	Biomacromolecules
Volume	18
Issue number	6
DOIs	https://doi.org/10.1021/acs.biomac.7b00294
Publication status	Published - Jun 2017
MoE publication type	A1 Journal article-refereed

Keywords

MIMETIC PROTEIN REC1-RESILIN
CELLULOSE-BINDING DOMAINS
TRICHODERMA-REESEI
POLYPROLINE-II
STIMULI
NANOCOMPOSITES
MECHANISM
MODULES

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10.1021/acs.biomac.7b00294

acs.biomac.7b00294
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title = "Elastic and pH-Responsive Hybrid Interfaces Created with Engineered Resilin and Nanocellulose",

abstract = "We investigated how a genetically engineered resilin fusion protein modifies cellulose surfaces. We characterized the pH-responsive behavior of a resilin-like polypeptide (RLP) having terminal cellulose binding modules (CBM) and showed its binding to cellulose nanofibrils (CNF). Characterization of the resilin fusion protein at different pHs revealed substantial conformational changes of the protein, which were observed as swelling and contraction of the protein layer bound to the nanocellulose surface. In addition, we showed that employment of the modified resilin in cellulose hydrogel and nanopaper increased their modulus of stiffness through a cross-linking effect.",

keywords = "MIMETIC PROTEIN REC1-RESILIN, CELLULOSE-BINDING DOMAINS, TRICHODERMA-REESEI, POLYPROLINE-II, STIMULI, NANOCOMPOSITES, MECHANISM, MODULES",

author = "Wenwen Fang and Arja Paananen and Marika Vitikainen and Salla Koskela and Ann Westerholm-Parvinen and Joensuu, {Jussi J.} and Landowski, {Christopher P.} and Merja Penttil{\"a} and Markus Linder and P{\"a}ivi Laaksonen",

year = "2017",

month = jun,

doi = "10.1021/acs.biomac.7b00294",

language = "English",

volume = "18",

pages = "1866--1873",

journal = "Biomacromolecules",

issn = "1525-7797",

publisher = "American Chemical Society",

number = "6",

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TY - JOUR

T1 - Elastic and pH-Responsive Hybrid Interfaces Created with Engineered Resilin and Nanocellulose

AU - Fang, Wenwen

AU - Paananen, Arja

AU - Vitikainen, Marika

AU - Koskela, Salla

AU - Westerholm-Parvinen, Ann

AU - Joensuu, Jussi J.

AU - Landowski, Christopher P.

AU - Penttilä, Merja

AU - Linder, Markus

AU - Laaksonen, Päivi

PY - 2017/6

Y1 - 2017/6

N2 - We investigated how a genetically engineered resilin fusion protein modifies cellulose surfaces. We characterized the pH-responsive behavior of a resilin-like polypeptide (RLP) having terminal cellulose binding modules (CBM) and showed its binding to cellulose nanofibrils (CNF). Characterization of the resilin fusion protein at different pHs revealed substantial conformational changes of the protein, which were observed as swelling and contraction of the protein layer bound to the nanocellulose surface. In addition, we showed that employment of the modified resilin in cellulose hydrogel and nanopaper increased their modulus of stiffness through a cross-linking effect.

AB - We investigated how a genetically engineered resilin fusion protein modifies cellulose surfaces. We characterized the pH-responsive behavior of a resilin-like polypeptide (RLP) having terminal cellulose binding modules (CBM) and showed its binding to cellulose nanofibrils (CNF). Characterization of the resilin fusion protein at different pHs revealed substantial conformational changes of the protein, which were observed as swelling and contraction of the protein layer bound to the nanocellulose surface. In addition, we showed that employment of the modified resilin in cellulose hydrogel and nanopaper increased their modulus of stiffness through a cross-linking effect.

KW - MIMETIC PROTEIN REC1-RESILIN

KW - CELLULOSE-BINDING DOMAINS

KW - TRICHODERMA-REESEI

KW - POLYPROLINE-II

KW - STIMULI

KW - NANOCOMPOSITES

KW - MECHANISM

KW - MODULES

U2 - 10.1021/acs.biomac.7b00294

DO - 10.1021/acs.biomac.7b00294

M3 - Article

SN - 1525-7797

VL - 18

SP - 1866

EP - 1873

JO - Biomacromolecules

JF - Biomacromolecules

IS - 6

ER -

Elastic and pH-Responsive Hybrid Interfaces Created with Engineered Resilin and Nanocellulose

Abstract

Keywords

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