Efficient biotransformation of cholesterol to androsta-1,4-diene-3,17-dione by a newly isolated actinomycete Gordonia neofelifaecis

Yuchang Liu, Guiying Chen, Fanglan Ge, Wei Li*, Lihua Zeng, Weiguo Cao

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

Abstract

A newly isolated actinomycete, Gordonia neofelifaecis (NRRL B-59395) from the faeces of Neofelis nebulosa, was used to selectively degrade the side-chain of cholesterol. The intermediates were purified and characterized. Quantitative analysis of the accumulated metabolites from cholesterol side-chain cleavage was conducted during the biotransformation. The results showed that the profile of accumulated intermediates was different from those of other reported microorganisms. Among the five metabolites, androsta-1,4-diene-3,17-dione (ADD) was the main product of the side-chain degradation, with a high conversion rate (87.2%), indicating its potential for industrial production of ADD. At the end of transformation, the substrate cholesterol was completely consumed. The effect of some factors on the bioconversion was also investigated. To our best knowledge, this is the first report regarding cholesterol side-chain cleavage using bacteria belonging to Gordonia.

Original languageEnglish
Pages (from-to)759-765
Number of pages7
JournalWORLD JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
Volume27
Issue number4
DOIs
Publication statusPublished - Apr 2011
MoE publication typeA1 Journal article-refereed

Keywords

  • Gordonia neofelifaecis
  • Side-chain cleavage of cholesterol
  • Androsta-1,4-diene-3,17-dione
  • MICROBIAL TRANSFORMATION
  • DEGRADING ACTINOMYCETE
  • SP NOV.
  • STEROLS
  • TESTOSTERONE
  • PHYTOSTEROL
  • DEGRADATION
  • KETONES

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