EFFECTS OF LIGNIN ON THE HYDROLYSIS OF CELLULOSE BY PURE AND MULTICOMPONENT ENZYMES

Raquel Martin-Sampedro, Jenni Rahikainen, Ingrid Hoeger, Kaisa Marjamaa, Kristiina Kruus, Ilari Filpponen, Janne Laine, Orlando J. Rojas*

*Corresponding author for this work

    Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

    Abstract

    The utilization of lignocellulose in second generation bioethanol requires pre-treatment technologies to break down the various physical and chemical barriers that inhibit the susceptibility for bioprocessing (including recalcitrance, cellulose access, etc.). Therefore, enzymatic hydrolysis of cellulose and the influence of lignin on bioconversion are critical in making any process viable.

    Due to the complexity of lignocellulose systems most studies in this area have measured the overall production of sugars upon enzymatic hydrolysis of suspended substrates. However, the effect of lignin on cellulolytic reactions has not been addressed at the molecular and nano scales. Therefore, the objective of this work is to investigate the role of lignin on the dynamics and efficiency of biocatalytic conversion of cellulose by carrying out in-situ, real time quartz crystal microgravimetry experiments. To this end, novel bi-component thin films containing cellulose and lignin were employed.

    A commercial multicomponent enzyme system as well as endoglucanase, EG I and exoglucanase, CBH I from Trichoderma reesei were used. It was found that both enzymes adsorbed onto neat cellulose or lignin films, although EG I exhibited a higher affinity to lignin. Due to the adsorption of enzyme onto lignin, a clear reduction in the hydrolysis rate was observed in the presence of lignin.

    Original languageEnglish
    Title of host publicationPROCEEDING OF THE 4TH INTERNATIONAL CONFERENCE ON PULPING, PAPERMAKING AND BIOTECHNOLOGY (ICPPB '12), VOLS. I AND II
    EditorsY Jin, Z Wang, W Wu
    PublisherNanjing Forestry University
    Pages1044-1049
    Number of pages6
    ISBN (Print)978-7-5019-9043-6
    Publication statusPublished - 2012
    MoE publication typeA4 Conference publication
    EventInternational Conference on Pulping, Papermaking and Biotechnology - Nanjing, China
    Duration: 7 Nov 20129 Nov 2012
    Conference number: 4

    Conference

    ConferenceInternational Conference on Pulping, Papermaking and Biotechnology
    Abbreviated titleICPPB
    Country/TerritoryChina
    CityNanjing
    Period07/11/201209/11/2012

    Keywords

    • lignin inhibition
    • cellulose enzymatic hydrolysis
    • endoglucanase EG I
    • exoglucanase CBH I
    • Trichoderma reesei
    • QUARTZ-CRYSTAL MICROBALANCE
    • ENZYMATIC-HYDROLYSIS
    • INHIBITION
    • RECALCITRANCE
    • DEGRADATION
    • SURFACTANT
    • SOFTWOOD
    • DOMAINS
    • FIBER

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