EFFECTS OF LIGNIN ON THE HYDROLYSIS OF CELLULOSE BY PURE AND MULTICOMPONENT ENZYMES

Raquel Martin-Sampedro; Jenni Rahikainen; Ingrid Hoeger; Kaisa Marjamaa; Kristiina Kruus; Ilari Filpponen; Janne Laine; Orlando J. Rojas

EFFECTS OF LIGNIN ON THE HYDROLYSIS OF CELLULOSE BY PURE AND MULTICOMPONENT ENZYMES

Raquel Martin-Sampedro, Jenni Rahikainen, Ingrid Hoeger, Kaisa Marjamaa, Kristiina Kruus, Ilari Filpponen, Janne Laine, Orlando J. Rojas^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Scientific › peer-review

Abstract

The utilization of lignocellulose in second generation bioethanol requires pre-treatment technologies to break down the various physical and chemical barriers that inhibit the susceptibility for bioprocessing (including recalcitrance, cellulose access, etc.). Therefore, enzymatic hydrolysis of cellulose and the influence of lignin on bioconversion are critical in making any process viable.

Due to the complexity of lignocellulose systems most studies in this area have measured the overall production of sugars upon enzymatic hydrolysis of suspended substrates. However, the effect of lignin on cellulolytic reactions has not been addressed at the molecular and nano scales. Therefore, the objective of this work is to investigate the role of lignin on the dynamics and efficiency of biocatalytic conversion of cellulose by carrying out in-situ, real time quartz crystal microgravimetry experiments. To this end, novel bi-component thin films containing cellulose and lignin were employed.

A commercial multicomponent enzyme system as well as endoglucanase, EG I and exoglucanase, CBH I from Trichoderma reesei were used. It was found that both enzymes adsorbed onto neat cellulose or lignin films, although EG I exhibited a higher affinity to lignin. Due to the adsorption of enzyme onto lignin, a clear reduction in the hydrolysis rate was observed in the presence of lignin.

Original language	English
Title of host publication	PROCEEDING OF THE 4TH INTERNATIONAL CONFERENCE ON PULPING, PAPERMAKING AND BIOTECHNOLOGY (ICPPB '12), VOLS. I AND II
Editors	Y Jin, Z Wang, W Wu
Publisher	Nanjing Forestry University
Pages	1044-1049
Number of pages	6
ISBN (Print)	978-7-5019-9043-6
Publication status	Published - 2012
MoE publication type	A4 Article in a conference publication
Event	International Conference on Pulping, Papermaking and Biotechnology - Nanjing, China Duration: 7 Nov 2012 → 9 Nov 2012 Conference number: 4

Conference

Conference	International Conference on Pulping, Papermaking and Biotechnology
Abbreviated title	ICPPB
Country/Territory	China
City	Nanjing
Period	07/11/2012 → 09/11/2012

Keywords

lignin inhibition
cellulose enzymatic hydrolysis
endoglucanase EG I
exoglucanase CBH I
Trichoderma reesei
QUARTZ-CRYSTAL MICROBALANCE
ENZYMATIC-HYDROLYSIS
INHIBITION
RECALCITRANCE
DEGRADATION
SURFACTANT
SOFTWOOD
DOMAINS
FIBER

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Martin-Sampedro, R., Rahikainen, J., Hoeger, I., Marjamaa, K., Kruus, K., Filpponen, I., Laine, J., & Rojas, O. J. (2012). EFFECTS OF LIGNIN ON THE HYDROLYSIS OF CELLULOSE BY PURE AND MULTICOMPONENT ENZYMES. In Y. Jin, Z. Wang, & W. Wu (Eds.), PROCEEDING OF THE 4TH INTERNATIONAL CONFERENCE ON PULPING, PAPERMAKING AND BIOTECHNOLOGY (ICPPB '12), VOLS. I AND II (pp. 1044-1049). Nanjing Forestry University.

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abstract = "The utilization of lignocellulose in second generation bioethanol requires pre-treatment technologies to break down the various physical and chemical barriers that inhibit the susceptibility for bioprocessing (including recalcitrance, cellulose access, etc.). Therefore, enzymatic hydrolysis of cellulose and the influence of lignin on bioconversion are critical in making any process viable.Due to the complexity of lignocellulose systems most studies in this area have measured the overall production of sugars upon enzymatic hydrolysis of suspended substrates. However, the effect of lignin on cellulolytic reactions has not been addressed at the molecular and nano scales. Therefore, the objective of this work is to investigate the role of lignin on the dynamics and efficiency of biocatalytic conversion of cellulose by carrying out in-situ, real time quartz crystal microgravimetry experiments. To this end, novel bi-component thin films containing cellulose and lignin were employed.A commercial multicomponent enzyme system as well as endoglucanase, EG I and exoglucanase, CBH I from Trichoderma reesei were used. It was found that both enzymes adsorbed onto neat cellulose or lignin films, although EG I exhibited a higher affinity to lignin. Due to the adsorption of enzyme onto lignin, a clear reduction in the hydrolysis rate was observed in the presence of lignin.",

keywords = "lignin inhibition, cellulose enzymatic hydrolysis, endoglucanase EG I, exoglucanase CBH I, Trichoderma reesei, QUARTZ-CRYSTAL MICROBALANCE, ENZYMATIC-HYDROLYSIS, INHIBITION, RECALCITRANCE, DEGRADATION, SURFACTANT, SOFTWOOD, DOMAINS, FIBER",

author = "Raquel Martin-Sampedro and Jenni Rahikainen and Ingrid Hoeger and Kaisa Marjamaa and Kristiina Kruus and Ilari Filpponen and Janne Laine and Rojas, {Orlando J.}",

year = "2012",

language = "English",

isbn = "978-7-5019-9043-6",

pages = "1044--1049",

editor = "Y Jin and Z Wang and W Wu",

booktitle = "PROCEEDING OF THE 4TH INTERNATIONAL CONFERENCE ON PULPING, PAPERMAKING AND BIOTECHNOLOGY (ICPPB '12), VOLS. I AND II",

publisher = "Nanjing Forestry University",

address = "China",

note = "International Conference on Pulping, Papermaking and Biotechnology, ICPPB ; Conference date: 07-11-2012 Through 09-11-2012",

}

Martin-Sampedro, R, Rahikainen, J, Hoeger, I, Marjamaa, K, Kruus, K, Filpponen, I, Laine, J & Rojas, OJ 2012, EFFECTS OF LIGNIN ON THE HYDROLYSIS OF CELLULOSE BY PURE AND MULTICOMPONENT ENZYMES. in Y Jin, Z Wang & W Wu (eds), PROCEEDING OF THE 4TH INTERNATIONAL CONFERENCE ON PULPING, PAPERMAKING AND BIOTECHNOLOGY (ICPPB '12), VOLS. I AND II. Nanjing Forestry University, pp. 1044-1049, International Conference on Pulping, Papermaking and Biotechnology, Nanjing, China, 07/11/2012.

EFFECTS OF LIGNIN ON THE HYDROLYSIS OF CELLULOSE BY PURE AND MULTICOMPONENT ENZYMES. / Martin-Sampedro, Raquel; Rahikainen, Jenni; Hoeger, Ingrid et al.

PROCEEDING OF THE 4TH INTERNATIONAL CONFERENCE ON PULPING, PAPERMAKING AND BIOTECHNOLOGY (ICPPB '12), VOLS. I AND II. ed. / Y Jin; Z Wang; W Wu. Nanjing Forestry University, 2012. p. 1044-1049.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Scientific › peer-review

TY - GEN

T1 - EFFECTS OF LIGNIN ON THE HYDROLYSIS OF CELLULOSE BY PURE AND MULTICOMPONENT ENZYMES

AU - Martin-Sampedro, Raquel

AU - Rahikainen, Jenni

AU - Hoeger, Ingrid

AU - Marjamaa, Kaisa

AU - Kruus, Kristiina

AU - Filpponen, Ilari

AU - Laine, Janne

AU - Rojas, Orlando J.

N1 - Conference code: 4

PY - 2012

Y1 - 2012

N2 - The utilization of lignocellulose in second generation bioethanol requires pre-treatment technologies to break down the various physical and chemical barriers that inhibit the susceptibility for bioprocessing (including recalcitrance, cellulose access, etc.). Therefore, enzymatic hydrolysis of cellulose and the influence of lignin on bioconversion are critical in making any process viable.Due to the complexity of lignocellulose systems most studies in this area have measured the overall production of sugars upon enzymatic hydrolysis of suspended substrates. However, the effect of lignin on cellulolytic reactions has not been addressed at the molecular and nano scales. Therefore, the objective of this work is to investigate the role of lignin on the dynamics and efficiency of biocatalytic conversion of cellulose by carrying out in-situ, real time quartz crystal microgravimetry experiments. To this end, novel bi-component thin films containing cellulose and lignin were employed.A commercial multicomponent enzyme system as well as endoglucanase, EG I and exoglucanase, CBH I from Trichoderma reesei were used. It was found that both enzymes adsorbed onto neat cellulose or lignin films, although EG I exhibited a higher affinity to lignin. Due to the adsorption of enzyme onto lignin, a clear reduction in the hydrolysis rate was observed in the presence of lignin.

AB - The utilization of lignocellulose in second generation bioethanol requires pre-treatment technologies to break down the various physical and chemical barriers that inhibit the susceptibility for bioprocessing (including recalcitrance, cellulose access, etc.). Therefore, enzymatic hydrolysis of cellulose and the influence of lignin on bioconversion are critical in making any process viable.Due to the complexity of lignocellulose systems most studies in this area have measured the overall production of sugars upon enzymatic hydrolysis of suspended substrates. However, the effect of lignin on cellulolytic reactions has not been addressed at the molecular and nano scales. Therefore, the objective of this work is to investigate the role of lignin on the dynamics and efficiency of biocatalytic conversion of cellulose by carrying out in-situ, real time quartz crystal microgravimetry experiments. To this end, novel bi-component thin films containing cellulose and lignin were employed.A commercial multicomponent enzyme system as well as endoglucanase, EG I and exoglucanase, CBH I from Trichoderma reesei were used. It was found that both enzymes adsorbed onto neat cellulose or lignin films, although EG I exhibited a higher affinity to lignin. Due to the adsorption of enzyme onto lignin, a clear reduction in the hydrolysis rate was observed in the presence of lignin.

KW - lignin inhibition

KW - cellulose enzymatic hydrolysis

KW - endoglucanase EG I

KW - exoglucanase CBH I

KW - Trichoderma reesei

KW - QUARTZ-CRYSTAL MICROBALANCE

KW - ENZYMATIC-HYDROLYSIS

KW - INHIBITION

KW - RECALCITRANCE

KW - DEGRADATION

KW - SURFACTANT

KW - SOFTWOOD

KW - DOMAINS

KW - FIBER

M3 - Conference contribution

SN - 978-7-5019-9043-6

SP - 1044

EP - 1049

BT - PROCEEDING OF THE 4TH INTERNATIONAL CONFERENCE ON PULPING, PAPERMAKING AND BIOTECHNOLOGY (ICPPB '12), VOLS. I AND II

A2 - Jin, Y

A2 - Wang, Z

A2 - Wu, W

PB - Nanjing Forestry University

T2 - International Conference on Pulping, Papermaking and Biotechnology

Y2 - 7 November 2012 through 9 November 2012

ER -

EFFECTS OF LIGNIN ON THE HYDROLYSIS OF CELLULOSE BY PURE AND MULTICOMPONENT ENZYMES

Abstract

Conference

Keywords

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