Effect of gamma irradiation and heat treatment on the artificial contamination of maize grains by Aspergillus flavus Link NRRL 5906

Research output: Contribution to journalArticle


  • Mohamed Albuzaudi
  • Tero Eerikäinen

  • Ossi Turunen
  • Mohamed Ghelawi
  • M. El Haj Assad
  • M. Tawalbeh
  • Dattatray Bedade
  • Salem Shamekh

Research units

  • Biotechnology Research Center
  • Institute of Chemical Technology
  • University of Sharjah


Maize (Zea mays L.) is one of the main crops, which is easily susceptable to Aspergillus flavus infection resulting in huge losses worldwide. This study was carried out to investigate the effect of combining heat and irradiation treatments in controlling the fungal growth in maize grains. Surface disinfected maize grains were artificially contaminated with spores of Aspergillus flavus Link NRRL 5906, and then exposed to gamma radiation with doses of 3.0, 4.0 and 5.0 kGy. The samples were additionally heat treated at 60 °C for 30 min. The heat and irradiation treatments showed a synergistic effect on controlling Aspergillus flavus growth. The heat treatment reduced the required radiation dose of about 0.5–1.0 kGy when 4.0 kGy or 5.0 kGy irradiation was used. The combined heat and irradiation treatment of moisture reduced the average CFU by 8 log cycles when 4 kGy or 5 kGy irradiation was used and by 7 log cycles when 3 kGy irradiation was used. The heat treatment of moisture alone reduced the average CFU by only by 0.8 log cycles. Combining irradiation with heat treatment to reduce the required radiation dose is very useful especially when there is a concern over biological side effects of irradiation.


Original languageEnglish
Pages (from-to)57-63
Number of pages7
Publication statusPublished - 1 Mar 2017
MoE publication typeA1 Journal article-refereed

    Research areas

  • Aspergillus flavus, Gamma irradiation, Heat treatment, Maize

ID: 11091514