Effect of ciprofloxacin antibiotic on the partial-nitritation process and bacterial community structure of a submerged biofilter

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Effect of ciprofloxacin antibiotic on the partial-nitritation process and bacterial community structure of a submerged biofilter. / Gonzalez-Martinez, A.; Rodriguez-Sanchez, A.; Martinez-Toledo, M. V.; Garcia-Ruiz, M. J.; Hontoria, E.; Osorio-Robles, F.; Gonzalez-Lopez, J.

In: Science of the Total Environment, Vol. 476-477, 01.04.2014, p. 276-287.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Gonzalez-Martinez, A, Rodriguez-Sanchez, A, Martinez-Toledo, MV, Garcia-Ruiz, MJ, Hontoria, E, Osorio-Robles, F & Gonzalez-Lopez, J 2014, 'Effect of ciprofloxacin antibiotic on the partial-nitritation process and bacterial community structure of a submerged biofilter' Science of the Total Environment, vol. 476-477, pp. 276-287. https://doi.org/10.1016/j.scitotenv.2014.01.012

APA

Gonzalez-Martinez, A., Rodriguez-Sanchez, A., Martinez-Toledo, M. V., Garcia-Ruiz, M. J., Hontoria, E., Osorio-Robles, F., & Gonzalez-Lopez, J. (2014). Effect of ciprofloxacin antibiotic on the partial-nitritation process and bacterial community structure of a submerged biofilter. Science of the Total Environment, 476-477, 276-287. https://doi.org/10.1016/j.scitotenv.2014.01.012

Vancouver

Gonzalez-Martinez A, Rodriguez-Sanchez A, Martinez-Toledo MV, Garcia-Ruiz MJ, Hontoria E, Osorio-Robles F et al. Effect of ciprofloxacin antibiotic on the partial-nitritation process and bacterial community structure of a submerged biofilter. Science of the Total Environment. 2014 Apr 1;476-477:276-287. https://doi.org/10.1016/j.scitotenv.2014.01.012

Author

Gonzalez-Martinez, A. ; Rodriguez-Sanchez, A. ; Martinez-Toledo, M. V. ; Garcia-Ruiz, M. J. ; Hontoria, E. ; Osorio-Robles, F. ; Gonzalez-Lopez, J. / Effect of ciprofloxacin antibiotic on the partial-nitritation process and bacterial community structure of a submerged biofilter. In: Science of the Total Environment. 2014 ; Vol. 476-477. pp. 276-287.

Bibtex - Download

@article{81bbb4f3aeb549b8acde5a065fd897e8,
title = "Effect of ciprofloxacin antibiotic on the partial-nitritation process and bacterial community structure of a submerged biofilter",
abstract = "A partial-nitritation bench-scale submerged biofilter was used for the treatment of synthetic wastewater containing a high concentration of ammonium in order to study the influence of the antibiotic ciprofloxacin on the partial-nitritation process and biodiversity of the bacterial community structure. The influence of ciprofloxacin was evaluated in four partial-nitritation bioreactors working in parallel, which received sterile synthetic wastewater amended with 350. ng/L of ciprofloxacin (Experiment 1), synthetic wastewater without ciprofloxacin (Experiment 2), synthetic wastewater amended with 100. ng/L of ciprofloxacin (Experiment 3) and synthetic wastewater amended with 350. ng/L of ciprofloxacin (Experiment 4). The concentration of 100. ng/L of antibiotics demonstrated that the partial-nitritation process, microbial biomass and bacterial structure generated by tag-pyrosequencing adapted progressively to the conditions in the bioreactor. However, high concentrations of ciprofloxacin (350. ng/L) induced a decay of the partial-nitritation process, while the total microbial biomass was increased. Within the same experiment, the bacterial community experienced sequential shifts with a clear reduction of the ammonium oxidation bacteria (AOB) and an evident increase of Commamonas sp., which have been previously reported to be ciprofloxacin-resistant. Our study suggests the need for careful monitoring of the concentration of antibiotics such as ciprofloxacin in partial-nitritation bioreactors, in order to choose and maintain the most appropriate conditions for the proper operation of the system.",
keywords = "Antibiotics, Ciprofloxacin, Fluoroquinolone, Partial-nitritation, Pyrosequencing, Wastewater",
author = "A. Gonzalez-Martinez and A. Rodriguez-Sanchez and Martinez-Toledo, {M. V.} and Garcia-Ruiz, {M. J.} and E. Hontoria and F. Osorio-Robles and J. Gonzalez-Lopez",
year = "2014",
month = "4",
day = "1",
doi = "10.1016/j.scitotenv.2014.01.012",
language = "English",
volume = "476-477",
pages = "276--287",
journal = "Science of the Total Environment",
issn = "0048-9697",
publisher = "Elsevier Science B.V.",

}

RIS - Download

TY - JOUR

T1 - Effect of ciprofloxacin antibiotic on the partial-nitritation process and bacterial community structure of a submerged biofilter

AU - Gonzalez-Martinez, A.

AU - Rodriguez-Sanchez, A.

AU - Martinez-Toledo, M. V.

AU - Garcia-Ruiz, M. J.

AU - Hontoria, E.

AU - Osorio-Robles, F.

AU - Gonzalez-Lopez, J.

PY - 2014/4/1

Y1 - 2014/4/1

N2 - A partial-nitritation bench-scale submerged biofilter was used for the treatment of synthetic wastewater containing a high concentration of ammonium in order to study the influence of the antibiotic ciprofloxacin on the partial-nitritation process and biodiversity of the bacterial community structure. The influence of ciprofloxacin was evaluated in four partial-nitritation bioreactors working in parallel, which received sterile synthetic wastewater amended with 350. ng/L of ciprofloxacin (Experiment 1), synthetic wastewater without ciprofloxacin (Experiment 2), synthetic wastewater amended with 100. ng/L of ciprofloxacin (Experiment 3) and synthetic wastewater amended with 350. ng/L of ciprofloxacin (Experiment 4). The concentration of 100. ng/L of antibiotics demonstrated that the partial-nitritation process, microbial biomass and bacterial structure generated by tag-pyrosequencing adapted progressively to the conditions in the bioreactor. However, high concentrations of ciprofloxacin (350. ng/L) induced a decay of the partial-nitritation process, while the total microbial biomass was increased. Within the same experiment, the bacterial community experienced sequential shifts with a clear reduction of the ammonium oxidation bacteria (AOB) and an evident increase of Commamonas sp., which have been previously reported to be ciprofloxacin-resistant. Our study suggests the need for careful monitoring of the concentration of antibiotics such as ciprofloxacin in partial-nitritation bioreactors, in order to choose and maintain the most appropriate conditions for the proper operation of the system.

AB - A partial-nitritation bench-scale submerged biofilter was used for the treatment of synthetic wastewater containing a high concentration of ammonium in order to study the influence of the antibiotic ciprofloxacin on the partial-nitritation process and biodiversity of the bacterial community structure. The influence of ciprofloxacin was evaluated in four partial-nitritation bioreactors working in parallel, which received sterile synthetic wastewater amended with 350. ng/L of ciprofloxacin (Experiment 1), synthetic wastewater without ciprofloxacin (Experiment 2), synthetic wastewater amended with 100. ng/L of ciprofloxacin (Experiment 3) and synthetic wastewater amended with 350. ng/L of ciprofloxacin (Experiment 4). The concentration of 100. ng/L of antibiotics demonstrated that the partial-nitritation process, microbial biomass and bacterial structure generated by tag-pyrosequencing adapted progressively to the conditions in the bioreactor. However, high concentrations of ciprofloxacin (350. ng/L) induced a decay of the partial-nitritation process, while the total microbial biomass was increased. Within the same experiment, the bacterial community experienced sequential shifts with a clear reduction of the ammonium oxidation bacteria (AOB) and an evident increase of Commamonas sp., which have been previously reported to be ciprofloxacin-resistant. Our study suggests the need for careful monitoring of the concentration of antibiotics such as ciprofloxacin in partial-nitritation bioreactors, in order to choose and maintain the most appropriate conditions for the proper operation of the system.

KW - Antibiotics

KW - Ciprofloxacin

KW - Fluoroquinolone

KW - Partial-nitritation

KW - Pyrosequencing

KW - Wastewater

UR - http://www.scopus.com/inward/record.url?scp=84893003605&partnerID=8YFLogxK

U2 - 10.1016/j.scitotenv.2014.01.012

DO - 10.1016/j.scitotenv.2014.01.012

M3 - Article

VL - 476-477

SP - 276

EP - 287

JO - Science of the Total Environment

JF - Science of the Total Environment

SN - 0048-9697

ER -

ID: 8886004