Early detection of peripheral blood cell signature in children developing β-cell autoimmunity at a young age

Research output: Contribution to journalArticleScientificpeer-review

Researchers

  • Henna Kallionpää
  • Soile Tuomela
  • Ubaid Ullah
  • Rafael De Albuquerque
  • Tapio Lönnberg
  • Elina Komsi
  • Heli Siljander
  • Jarno Honkanen
  • Taina Härkönen
  • Aleksandr Peet
  • Vallo Tillmann
  • Vikash Chandra
  • Mahesh Kumar Anagandula
  • Gun Frisk
  • Timo Otonkoski
  • Omid Rasool
  • Riikka Lund
  • Mikael Knip
  • Riitta Lahesmaa

Research units

  • University of Turku
  • University of Helsinki
  • University of Tartu
  • Uppsala University
  • Folkhalsan
  • Tampere University Hospital

Abstract

The appearance of type 1 diabetes (T1D)-associated autoantibodies is the first and only measurable parameter to predict progression toward T1D in genetically susceptible individuals. However, autoantibodies indicate an active autoimmune reaction, wherein the immune tolerance is already broken. Therefore, there is a clear and urgent need for new biomarkers that predict the onset of the autoimmune reaction preceding autoantibody positivity or reflect progressive β-cell destruction. Here we report the mRNA sequencing-based analysis of 306 samples including fractionated samples of CD4+ and CD8+ T cells as well as CD4-CD8- cell fractions and unfractionated peripheral blood mononuclear cell samples longitudinally collected from seven children who developed β-cell autoimmunity (case subjects) at a young age and matched control subjects. We identified transcripts, including interleukin 32 (IL32), that were upregulated before T1D-associated autoantibodies appeared. Single-cell RNA sequencing studies revealed that high IL32 in case samples was contributed mainly by activated T cells and NK cells. Further, we showed that IL32 expression can be induced by a virus and cytokines in pancreatic islets and β-cells, respectively. The results provide a basis for early detection of aberrations in the immune system function before T1D and suggest a potential role for IL32 in the pathogenesis of T1D.

Details

Original languageEnglish
Pages (from-to)2024-2034
Number of pages11
JournalDIABETES
Volume68
Issue number10
Publication statusPublished - 1 Oct 2019
MoE publication typeA1 Journal article-refereed

ID: 37377955