Diversity of ribosomal 16S DNA- and RNA-based bacterial community in an office building drinking water system

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Diversity of ribosomal 16S DNA- and RNA-based bacterial community in an office building drinking water system. / Inkinen, J.; Jayaprakash, B.; Santo Domingo, J. W.; Keinänen-Toivola, M. M.; Ryu, H.; Pitkänen, T.

In: Journal of Applied Microbiology, Vol. 120, No. 6, 2016, p. 1723–1738 .

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Inkinen, J, Jayaprakash, B, Santo Domingo, JW, Keinänen-Toivola, MM, Ryu, H & Pitkänen, T 2016, 'Diversity of ribosomal 16S DNA- and RNA-based bacterial community in an office building drinking water system' Journal of Applied Microbiology, vol. 120, no. 6, pp. 1723–1738 . https://doi.org/10.1111/jam.13144

APA

Inkinen, J., Jayaprakash, B., Santo Domingo, J. W., Keinänen-Toivola, M. M., Ryu, H., & Pitkänen, T. (2016). Diversity of ribosomal 16S DNA- and RNA-based bacterial community in an office building drinking water system. Journal of Applied Microbiology, 120(6), 1723–1738 . https://doi.org/10.1111/jam.13144

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Author

Inkinen, J. ; Jayaprakash, B. ; Santo Domingo, J. W. ; Keinänen-Toivola, M. M. ; Ryu, H. ; Pitkänen, T. / Diversity of ribosomal 16S DNA- and RNA-based bacterial community in an office building drinking water system. In: Journal of Applied Microbiology. 2016 ; Vol. 120, No. 6. pp. 1723–1738 .

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@article{59a396e8228e4aa293e14985f28ed0af,
title = "Diversity of ribosomal 16S DNA- and RNA-based bacterial community in an office building drinking water system",
abstract = "Aims: Next-generation sequencing of 16S ribosomal RNA genes (rDNA) and ribosomal RNA (rRNA) was used to characterize water and biofilm microbiome collected from a drinking water distribution system of an office building after its first year of operation. Methods and Results: The total bacterial community (rDNA) and active bacterial members (rRNA) sequencing databases were generated by Illumina MiSeq PE250 platform. As estimated by Chao1 index, species richness in cold water system was lower (180-260) in biofilms (Sphingomonas spp., Methylobacterium spp., Limnohabitans spp., Rhizobiales order) than in waters (250-580), (also Methylotenera spp.) (P = 0·005, n = 20). Similarly species richness (Chao1) was slightly higher (210-580) in rDNA libraries compared to rRNA libraries (150-400; P = 0·054, n = 24). Active Mycobacterium spp. was found in cross-linked polyethylene (PEX), but not in corresponding copper pipeline biofilm. Nonpathogenic Legionella spp. was found in rDNA libraries but not in rRNA libraries. Conclusions: Microbial communities differed between water and biofilms, between cold and hot water systems, locations in the building and between water rRNA and rDNA libraries, as shown by clear clusters in principal component analysis (PcoA). By using the rRNA method, we found that not all bacterial community members were active (e.g. Legionella spp.), whereas other members showed increased activity in some locations; for example, Pseudomonas spp. in hot water circulations' biofilm and order Rhizobiales and Limnohabitans spp. in stagnated locations' water and biofilm. Significance and Impact of the Study: rRNA-based methods may be better than rDNA-based methods for evaluating human health implications as rRNA methods can be used to describe the active bacterial fraction. This study indicates that copper as a pipeline material might have an adverse impact on the occurrence of Mycobacterium spp. The activity of Legionella spp. maybe questionable when detected solely by using DNA-based methods.",
keywords = "Biofilms, Drinking water, Microbial community, Next-generation sequencing, Pipeline network",
author = "J. Inkinen and B. Jayaprakash and {Santo Domingo}, {J. W.} and Kein{\"a}nen-Toivola, {M. M.} and H. Ryu and T. Pitk{\"a}nen",
year = "2016",
doi = "10.1111/jam.13144",
language = "English",
volume = "120",
pages = "1723–1738",
journal = "Journal of Applied Microbiology",
issn = "1364-5072",
publisher = "Wiley",
number = "6",

}

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TY - JOUR

T1 - Diversity of ribosomal 16S DNA- and RNA-based bacterial community in an office building drinking water system

AU - Inkinen, J.

AU - Jayaprakash, B.

AU - Santo Domingo, J. W.

AU - Keinänen-Toivola, M. M.

AU - Ryu, H.

AU - Pitkänen, T.

PY - 2016

Y1 - 2016

N2 - Aims: Next-generation sequencing of 16S ribosomal RNA genes (rDNA) and ribosomal RNA (rRNA) was used to characterize water and biofilm microbiome collected from a drinking water distribution system of an office building after its first year of operation. Methods and Results: The total bacterial community (rDNA) and active bacterial members (rRNA) sequencing databases were generated by Illumina MiSeq PE250 platform. As estimated by Chao1 index, species richness in cold water system was lower (180-260) in biofilms (Sphingomonas spp., Methylobacterium spp., Limnohabitans spp., Rhizobiales order) than in waters (250-580), (also Methylotenera spp.) (P = 0·005, n = 20). Similarly species richness (Chao1) was slightly higher (210-580) in rDNA libraries compared to rRNA libraries (150-400; P = 0·054, n = 24). Active Mycobacterium spp. was found in cross-linked polyethylene (PEX), but not in corresponding copper pipeline biofilm. Nonpathogenic Legionella spp. was found in rDNA libraries but not in rRNA libraries. Conclusions: Microbial communities differed between water and biofilms, between cold and hot water systems, locations in the building and between water rRNA and rDNA libraries, as shown by clear clusters in principal component analysis (PcoA). By using the rRNA method, we found that not all bacterial community members were active (e.g. Legionella spp.), whereas other members showed increased activity in some locations; for example, Pseudomonas spp. in hot water circulations' biofilm and order Rhizobiales and Limnohabitans spp. in stagnated locations' water and biofilm. Significance and Impact of the Study: rRNA-based methods may be better than rDNA-based methods for evaluating human health implications as rRNA methods can be used to describe the active bacterial fraction. This study indicates that copper as a pipeline material might have an adverse impact on the occurrence of Mycobacterium spp. The activity of Legionella spp. maybe questionable when detected solely by using DNA-based methods.

AB - Aims: Next-generation sequencing of 16S ribosomal RNA genes (rDNA) and ribosomal RNA (rRNA) was used to characterize water and biofilm microbiome collected from a drinking water distribution system of an office building after its first year of operation. Methods and Results: The total bacterial community (rDNA) and active bacterial members (rRNA) sequencing databases were generated by Illumina MiSeq PE250 platform. As estimated by Chao1 index, species richness in cold water system was lower (180-260) in biofilms (Sphingomonas spp., Methylobacterium spp., Limnohabitans spp., Rhizobiales order) than in waters (250-580), (also Methylotenera spp.) (P = 0·005, n = 20). Similarly species richness (Chao1) was slightly higher (210-580) in rDNA libraries compared to rRNA libraries (150-400; P = 0·054, n = 24). Active Mycobacterium spp. was found in cross-linked polyethylene (PEX), but not in corresponding copper pipeline biofilm. Nonpathogenic Legionella spp. was found in rDNA libraries but not in rRNA libraries. Conclusions: Microbial communities differed between water and biofilms, between cold and hot water systems, locations in the building and between water rRNA and rDNA libraries, as shown by clear clusters in principal component analysis (PcoA). By using the rRNA method, we found that not all bacterial community members were active (e.g. Legionella spp.), whereas other members showed increased activity in some locations; for example, Pseudomonas spp. in hot water circulations' biofilm and order Rhizobiales and Limnohabitans spp. in stagnated locations' water and biofilm. Significance and Impact of the Study: rRNA-based methods may be better than rDNA-based methods for evaluating human health implications as rRNA methods can be used to describe the active bacterial fraction. This study indicates that copper as a pipeline material might have an adverse impact on the occurrence of Mycobacterium spp. The activity of Legionella spp. maybe questionable when detected solely by using DNA-based methods.

KW - Biofilms

KW - Drinking water

KW - Microbial community

KW - Next-generation sequencing

KW - Pipeline network

UR - http://www.scopus.com/inward/record.url?scp=84964334323&partnerID=8YFLogxK

U2 - 10.1111/jam.13144

DO - 10.1111/jam.13144

M3 - Article

VL - 120

SP - 1723

EP - 1738

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

SN - 1364-5072

IS - 6

ER -

ID: 3350291