Dissecting the effect of chemical additives on the enzymatic hydrolysis of pretreated wheat straw

Research output: Contribution to journalArticleScientificpeer-review


Research units

  • Clariant
  • Graz University of Technology
  • Austrian Centre of Industrial Biotechnology


Chemical additives were examined for ability to increase the enzymatic hydrolysis of thermo-acidically pretreated wheat straw by Trichoderma reesei cellulase at 50°C. Semi-empirical descriptors derived from the hydrolysis time courses were applied to compare influence of these additives on lignocellulose bioconversion on a kinetic level, presenting a novel view on their mechanism of action. Focus was on rate retardation during hydrolysis, substrate conversion and enzyme adsorption. PEG 8000 enabled a reduction of enzyme loading by 50% while retaining the same conversion of 67% after 24h. For the first time, a beneficial effect of urea is reported, increasing the final substrate conversion after 48h by 16%. The cationic surfactant cetyl-trimethylammonium bromide (CTAB) enhanced the hydrolysis rate at extended reaction time (rlim) by 34% and reduced reaction time by 28%. A combination of PEG 8000 and urea increased sugar release more than additives used individually.


Original languageEnglish
Pages (from-to)713-722
Number of pages10
JournalBioresource Technology
Publication statusPublished - 2014
MoE publication typeA1 Journal article-refereed

    Research areas

  • Cellulase, Chemical additives, Enzymatic hydrolysis, Protein adsorption, Urea

ID: 13017918