TY - JOUR
T1 - Dissecting the effect of chemical additives on the enzymatic hydrolysis of pretreated wheat straw
AU - Monschein, Mareike
AU - Reisinger, Christoph
AU - Nidetzky, Bernd
PY - 2014
Y1 - 2014
N2 - Chemical additives were examined for ability to increase the enzymatic hydrolysis of thermo-acidically pretreated wheat straw by Trichoderma reesei cellulase at 50°C. Semi-empirical descriptors derived from the hydrolysis time courses were applied to compare influence of these additives on lignocellulose bioconversion on a kinetic level, presenting a novel view on their mechanism of action. Focus was on rate retardation during hydrolysis, substrate conversion and enzyme adsorption. PEG 8000 enabled a reduction of enzyme loading by 50% while retaining the same conversion of 67% after 24h. For the first time, a beneficial effect of urea is reported, increasing the final substrate conversion after 48h by 16%. The cationic surfactant cetyl-trimethylammonium bromide (CTAB) enhanced the hydrolysis rate at extended reaction time (rlim) by 34% and reduced reaction time by 28%. A combination of PEG 8000 and urea increased sugar release more than additives used individually.
AB - Chemical additives were examined for ability to increase the enzymatic hydrolysis of thermo-acidically pretreated wheat straw by Trichoderma reesei cellulase at 50°C. Semi-empirical descriptors derived from the hydrolysis time courses were applied to compare influence of these additives on lignocellulose bioconversion on a kinetic level, presenting a novel view on their mechanism of action. Focus was on rate retardation during hydrolysis, substrate conversion and enzyme adsorption. PEG 8000 enabled a reduction of enzyme loading by 50% while retaining the same conversion of 67% after 24h. For the first time, a beneficial effect of urea is reported, increasing the final substrate conversion after 48h by 16%. The cationic surfactant cetyl-trimethylammonium bromide (CTAB) enhanced the hydrolysis rate at extended reaction time (rlim) by 34% and reduced reaction time by 28%. A combination of PEG 8000 and urea increased sugar release more than additives used individually.
KW - Cellulase
KW - Chemical additives
KW - Enzymatic hydrolysis
KW - Protein adsorption
KW - Urea
UR - http://www.scopus.com/inward/record.url?scp=84907340177&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2014.07.054
DO - 10.1016/j.biortech.2014.07.054
M3 - Article
C2 - 25108473
AN - SCOPUS:84907340177
VL - 169
SP - 713
EP - 722
JO - Bioresource Technology
JF - Bioresource Technology
SN - 0960-8524
ER -