Disposable amperometric biosensor based on lactate oxidase immobilised on platinum nanoparticle-decorated carbon nanofiber and poly(diallyldimethylammonium chloride) films

Research output: Contribution to journalArticleScientificpeer-review

Researchers

  • Pedro J. Lamas-Ardisana
  • Oscar A. Loaiza
  • Larraitz Añorga
  • Elena Jubete
  • Maryam Borghei

  • Virginia Ruiz
  • Estibalitz Ochoteco
  • Germán Cabañero
  • Hans J. Grande

Research units

  • IK4 Cidetec

Abstract

A novel biosensor for lactate has been developed, using screen-printed carbon electrodes (SPCE) and lactate oxidase (LOx). The active surface of the electrodes was modified using a dispersion of platinum nanoparticle decorated carbon nanofibers (PtNp-CNF) in poly(diallyldimethylammonium) chloride (PDDA) solution. In this way, sensitive, disposable, low cost and reliable hydrogen peroxide sensors were obtained. The immobilisation of LOx on top of these PtNp-CNF-PDDA/SPCEs resulted in amperometric biosensors with high operational stability. The sensitivity of the optimised lactate biosensor was 36.8 (mA/Mcm2) with a linear range of 25-1500μM. The limit of detection was 11μM (S/N=3). Reproducibility, selectivity and storage stability were also evaluated. Additionally, the stability of the biosensor was also predicted by a model based on thermal degradation. Finally, lactate in sweat and blood samples was determined in a sport test using LOx/PtNp-CNF-PDDA/SPCEs and commercial biosensors respectively. Based on these data, the validity of the sweat lactate for the determination of the lactate threshold is discussed.

Details

Original languageEnglish
Pages (from-to)345-351
Number of pages7
JournalBiosensors and Bioelectronics
Volume56
Publication statusPublished - 15 Jun 2014
MoE publication typeA1 Journal article-refereed

    Research areas

  • Carbon nanofiber, Lactate, Lactate oxidase, Platinum nanoparticle, Screen-printed electrode, Sweat

ID: 9312719