TY - JOUR
T1 - Disposable amperometric biosensor based on lactate oxidase immobilised on platinum nanoparticle-decorated carbon nanofiber and poly(diallyldimethylammonium chloride) films
AU - Lamas-Ardisana, Pedro J.
AU - Loaiza, Oscar A.
AU - Añorga, Larraitz
AU - Jubete, Elena
AU - Borghei, Maryam
AU - Ruiz, Virginia
AU - Ochoteco, Estibalitz
AU - Cabañero, Germán
AU - Grande, Hans J.
PY - 2014/6/15
Y1 - 2014/6/15
N2 - A novel biosensor for lactate has been developed, using screen-printed carbon electrodes (SPCE) and lactate oxidase (LOx). The active surface of the electrodes was modified using a dispersion of platinum nanoparticle decorated carbon nanofibers (PtNp-CNF) in poly(diallyldimethylammonium) chloride (PDDA) solution. In this way, sensitive, disposable, low cost and reliable hydrogen peroxide sensors were obtained. The immobilisation of LOx on top of these PtNp-CNF-PDDA/SPCEs resulted in amperometric biosensors with high operational stability. The sensitivity of the optimised lactate biosensor was 36.8 (mA/Mcm2) with a linear range of 25-1500μM. The limit of detection was 11μM (S/N=3). Reproducibility, selectivity and storage stability were also evaluated. Additionally, the stability of the biosensor was also predicted by a model based on thermal degradation. Finally, lactate in sweat and blood samples was determined in a sport test using LOx/PtNp-CNF-PDDA/SPCEs and commercial biosensors respectively. Based on these data, the validity of the sweat lactate for the determination of the lactate threshold is discussed.
AB - A novel biosensor for lactate has been developed, using screen-printed carbon electrodes (SPCE) and lactate oxidase (LOx). The active surface of the electrodes was modified using a dispersion of platinum nanoparticle decorated carbon nanofibers (PtNp-CNF) in poly(diallyldimethylammonium) chloride (PDDA) solution. In this way, sensitive, disposable, low cost and reliable hydrogen peroxide sensors were obtained. The immobilisation of LOx on top of these PtNp-CNF-PDDA/SPCEs resulted in amperometric biosensors with high operational stability. The sensitivity of the optimised lactate biosensor was 36.8 (mA/Mcm2) with a linear range of 25-1500μM. The limit of detection was 11μM (S/N=3). Reproducibility, selectivity and storage stability were also evaluated. Additionally, the stability of the biosensor was also predicted by a model based on thermal degradation. Finally, lactate in sweat and blood samples was determined in a sport test using LOx/PtNp-CNF-PDDA/SPCEs and commercial biosensors respectively. Based on these data, the validity of the sweat lactate for the determination of the lactate threshold is discussed.
KW - Carbon nanofiber
KW - Lactate
KW - Lactate oxidase
KW - Platinum nanoparticle
KW - Screen-printed electrode
KW - Sweat
UR - http://www.scopus.com/inward/record.url?scp=84896696878&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2014.01.047
DO - 10.1016/j.bios.2014.01.047
M3 - Article
C2 - 24534552
AN - SCOPUS:84896696878
SN - 0956-5663
VL - 56
SP - 345
EP - 351
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
ER -