Discovery of novel secreted fungal sulfhydryl oxidases with a plate test screen

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Discovery of novel secreted fungal sulfhydryl oxidases with a plate test screen. / Nivala, Outi; Mattinen, Maija Liisa; Faccio, Greta; Buchert, Johanna; Kruus, Kristiina.

In: Applied Microbiology and Biotechnology, Vol. 97, No. 21, 01.11.2013, p. 9429-9437.

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Nivala, Outi ; Mattinen, Maija Liisa ; Faccio, Greta ; Buchert, Johanna ; Kruus, Kristiina. / Discovery of novel secreted fungal sulfhydryl oxidases with a plate test screen. In: Applied Microbiology and Biotechnology. 2013 ; Vol. 97, No. 21. pp. 9429-9437.

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@article{1287c1e5ff4347839a59e26ff448c851,
title = "Discovery of novel secreted fungal sulfhydryl oxidases with a plate test screen",
abstract = "Sulfhydryl oxidases (SOX) are FAD-dependent enzymes capable of oxidising free thiol groups and forming disulphide bonds. Although the quantity of scientific papers and suggested applications for SOX is constantly increasing, only a limited number of microbial SOX have been reported and are commercially available. Hence, the aim of this study was to develop a fast and reliable qualitative plate test for screening novel secreted fungal SOX. The screening was based on the Ellman's reagent, i.e. 5,5′-dithiobis[2-nitrobenzoic acid]. Altogether, 32 fungal strains from an in-house culture collection were screened. A total of 13 SOX-producing strains were found positive in the plate test screen. The novel SOX producers were Aspergillus tubingensis, Chaetomium globusum, Melanocarpus albomyces, Penicillium aurantiogriseum, Penicillium funiculosum, Coniophora puteana and Trametes hirsuta. Six of the discovered SOX were partially characterised by determination of isoelectric point, pH optimum and substrate specificity. A. tubingensis was identified as the most efficient novel SOX producer.",
keywords = "Ellman's reagent, Enzyme, Fungi, Oxidation, Screening, Sulfhydryl oxidase, Thiol",
author = "Outi Nivala and Mattinen, {Maija Liisa} and Greta Faccio and Johanna Buchert and Kristiina Kruus",
year = "2013",
month = "11",
day = "1",
doi = "10.1007/s00253-013-4753-9",
language = "English",
volume = "97",
pages = "9429--9437",
journal = "Applied Microbiology & Biotechnology",
issn = "0175-7598",
number = "21",

}

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TY - JOUR

T1 - Discovery of novel secreted fungal sulfhydryl oxidases with a plate test screen

AU - Nivala, Outi

AU - Mattinen, Maija Liisa

AU - Faccio, Greta

AU - Buchert, Johanna

AU - Kruus, Kristiina

PY - 2013/11/1

Y1 - 2013/11/1

N2 - Sulfhydryl oxidases (SOX) are FAD-dependent enzymes capable of oxidising free thiol groups and forming disulphide bonds. Although the quantity of scientific papers and suggested applications for SOX is constantly increasing, only a limited number of microbial SOX have been reported and are commercially available. Hence, the aim of this study was to develop a fast and reliable qualitative plate test for screening novel secreted fungal SOX. The screening was based on the Ellman's reagent, i.e. 5,5′-dithiobis[2-nitrobenzoic acid]. Altogether, 32 fungal strains from an in-house culture collection were screened. A total of 13 SOX-producing strains were found positive in the plate test screen. The novel SOX producers were Aspergillus tubingensis, Chaetomium globusum, Melanocarpus albomyces, Penicillium aurantiogriseum, Penicillium funiculosum, Coniophora puteana and Trametes hirsuta. Six of the discovered SOX were partially characterised by determination of isoelectric point, pH optimum and substrate specificity. A. tubingensis was identified as the most efficient novel SOX producer.

AB - Sulfhydryl oxidases (SOX) are FAD-dependent enzymes capable of oxidising free thiol groups and forming disulphide bonds. Although the quantity of scientific papers and suggested applications for SOX is constantly increasing, only a limited number of microbial SOX have been reported and are commercially available. Hence, the aim of this study was to develop a fast and reliable qualitative plate test for screening novel secreted fungal SOX. The screening was based on the Ellman's reagent, i.e. 5,5′-dithiobis[2-nitrobenzoic acid]. Altogether, 32 fungal strains from an in-house culture collection were screened. A total of 13 SOX-producing strains were found positive in the plate test screen. The novel SOX producers were Aspergillus tubingensis, Chaetomium globusum, Melanocarpus albomyces, Penicillium aurantiogriseum, Penicillium funiculosum, Coniophora puteana and Trametes hirsuta. Six of the discovered SOX were partially characterised by determination of isoelectric point, pH optimum and substrate specificity. A. tubingensis was identified as the most efficient novel SOX producer.

KW - Ellman's reagent

KW - Enzyme

KW - Fungi

KW - Oxidation

KW - Screening

KW - Sulfhydryl oxidase

KW - Thiol

UR - http://www.scopus.com/inward/record.url?scp=84885954912&partnerID=8YFLogxK

U2 - 10.1007/s00253-013-4753-9

DO - 10.1007/s00253-013-4753-9

M3 - Article

VL - 97

SP - 9429

EP - 9437

JO - Applied Microbiology & Biotechnology

JF - Applied Microbiology & Biotechnology

SN - 0175-7598

IS - 21

ER -

ID: 32948124