Discovery of novel secreted fungal sulfhydryl oxidases with a plate test screen

Research output: Contribution to journalArticle

Researchers

Research units

  • University of Helsinki
  • VTT Technical Research Centre of Finland
  • Swiss Federal Laboratories for Materials Science and Technology (Empa)

Abstract

Sulfhydryl oxidases (SOX) are FAD-dependent enzymes capable of oxidising free thiol groups and forming disulphide bonds. Although the quantity of scientific papers and suggested applications for SOX is constantly increasing, only a limited number of microbial SOX have been reported and are commercially available. Hence, the aim of this study was to develop a fast and reliable qualitative plate test for screening novel secreted fungal SOX. The screening was based on the Ellman's reagent, i.e. 5,5′-dithiobis[2-nitrobenzoic acid]. Altogether, 32 fungal strains from an in-house culture collection were screened. A total of 13 SOX-producing strains were found positive in the plate test screen. The novel SOX producers were Aspergillus tubingensis, Chaetomium globusum, Melanocarpus albomyces, Penicillium aurantiogriseum, Penicillium funiculosum, Coniophora puteana and Trametes hirsuta. Six of the discovered SOX were partially characterised by determination of isoelectric point, pH optimum and substrate specificity. A. tubingensis was identified as the most efficient novel SOX producer.

Details

Original languageEnglish
Pages (from-to)9429-9437
Number of pages9
JournalApplied Microbiology and Biotechnology
Volume97
Issue number21
Publication statusPublished - 1 Nov 2013
MoE publication typeA1 Journal article-refereed

    Research areas

  • Ellman's reagent, Enzyme, Fungi, Oxidation, Screening, Sulfhydryl oxidase, Thiol

ID: 32948124