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Developments in micro-and nano-defects detection using bacterial cells

  • Telmo G. Santos*
  • , R. M. Miranda
  • , M. Teresa Vieira
  • , A. Rita Farinha
  • , Telma J. Ferreira
  • , Luisa Quintino
  • , Pedro Santos Vilaca da Silva
  • , Carla C. C. R. de Carvalho
  • *Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

5 Citations (Web of Science)

Abstract

This paper describes improvements to the Nondestructive Testing (NDT) technique recently proposed, based on the use of bacterial cell suspensions to identify micro-and nano-surface defects. New bacterial strains were used with magnetic fields to improve bacteria mobility. Different materials and defect morphologies were tested, including nanoindentation defects, micro-powder injection moulding components and micro-laser welding. Nanoindentations with 0.6 mu m depth and 5.3 mu m side length were successfully detected. Bacterial cells allow identifying different topographic attributes of the surfaces, such as roughness. Cracks of about 0.5 mu m wide and 10 mu m depth in a reference test block Type 1 were successfully detected. (C) 2015 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)20-28
Number of pages9
JournalNDT & E International
Volume78
Issue numberMarch
DOIs
Publication statusPublished - Mar 2016
MoE publication typeA1 Journal article-refereed

Funding

The authors would like to acknowledge Fundacao para a Ciencia e a Tecnologia (FCT, I.P.) for funding the project "Defects Detection in Microfabrication With Bacterial Cells" - (MicroBac) (PTDC/EME-TME/118678/2010). TS and RM acknowledge PEst-OE/EME/UI0667/2014 and UID/EMS/00667/2013. C.C.C.R. de Carvalho acknowledges FCT for financial support under program "FCT Investigator 2013" (IF/01203/2013/CP1163/CT0002) and UID/BIO/04565/2013.

Keywords

  • Micro-surface defects
  • Bacterial cells
  • Magnetic field
  • Dye penetrant
  • STEEL

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