Developments in micro-and nano-defects detection using bacterial cells

Telmo G. Santos*, R. M. Miranda, M. Teresa Vieira, A. Rita Farinha, Telma J. Ferreira, Luisa Quintino, Pedro Santos Vilaca da Silva, Carla C. C. R. de Carvalho

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

4 Citations (Scopus)


This paper describes improvements to the Nondestructive Testing (NDT) technique recently proposed, based on the use of bacterial cell suspensions to identify micro-and nano-surface defects. New bacterial strains were used with magnetic fields to improve bacteria mobility. Different materials and defect morphologies were tested, including nanoindentation defects, micro-powder injection moulding components and micro-laser welding. Nanoindentations with 0.6 mu m depth and 5.3 mu m side length were successfully detected. Bacterial cells allow identifying different topographic attributes of the surfaces, such as roughness. Cracks of about 0.5 mu m wide and 10 mu m depth in a reference test block Type 1 were successfully detected. (C) 2015 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)20-28
Number of pages9
Issue numberMarch
Publication statusPublished - Mar 2016
MoE publication typeA1 Journal article-refereed


  • Micro-surface defects
  • Bacterial cells
  • Magnetic field
  • Dye penetrant


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