Developments in micro-and nano-defects detection using bacterial cells

Telmo G. Santos*, R. M. Miranda, M. Teresa Vieira, A. Rita Farinha, Telma J. Ferreira, Luisa Quintino, Pedro Santos Vilaca da Silva, Carla C. C. R. de Carvalho

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

3 Citations (Scopus)


This paper describes improvements to the Nondestructive Testing (NDT) technique recently proposed, based on the use of bacterial cell suspensions to identify micro-and nano-surface defects. New bacterial strains were used with magnetic fields to improve bacteria mobility. Different materials and defect morphologies were tested, including nanoindentation defects, micro-powder injection moulding components and micro-laser welding. Nanoindentations with 0.6 mu m depth and 5.3 mu m side length were successfully detected. Bacterial cells allow identifying different topographic attributes of the surfaces, such as roughness. Cracks of about 0.5 mu m wide and 10 mu m depth in a reference test block Type 1 were successfully detected. (C) 2015 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)20-28
Number of pages9
Issue numberMarch
Publication statusPublished - Mar 2016
MoE publication typeA1 Journal article-refereed


  • Micro-surface defects
  • Bacterial cells
  • Magnetic field
  • Dye penetrant

Cite this

Santos, T. G., Miranda, R. M., Teresa Vieira, M., Rita Farinha, A., Ferreira, T. J., Quintino, L., ... de Carvalho, C. C. C. R. (2016). Developments in micro-and nano-defects detection using bacterial cells. NDT & E INTERNATIONAL, 78(March), 20-28.