Determination of the Main Phase Transition Temperature of Phospholipids by Nanoplasmonic Sensing

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Determination of the Main Phase Transition Temperature of Phospholipids by Nanoplasmonic Sensing. / Chen, Wen; Duša, Filip; Witos, Joanna; Ruokonen, Suvi Katriina; Wiedmer, Susanne K.

In: Scientific Reports, Vol. 8, No. 1, 14815, 01.12.2018.

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Chen, Wen ; Duša, Filip ; Witos, Joanna ; Ruokonen, Suvi Katriina ; Wiedmer, Susanne K. / Determination of the Main Phase Transition Temperature of Phospholipids by Nanoplasmonic Sensing. In: Scientific Reports. 2018 ; Vol. 8, No. 1.

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@article{beb46f88de6c4f28bc446e701d8c07e2,
title = "Determination of the Main Phase Transition Temperature of Phospholipids by Nanoplasmonic Sensing",
abstract = "Our study demonstrates that nanoplasmonic sensing (NPS) can be utilized for the determination of the phase transition temperature (Tm) of phospholipids. During the phase transition, the lipid bilayer undergoes a conformational change. Therefore, it is presumed that the Tm of phospholipids can be determined by detecting conformational changes in liposomes. The studied lipids included 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC). Liposomes in gel phase are immobilized onto silicon dioxide sensors and the sensor cell temperature is increased until passing the Tm of the lipid. The results show that, when the system temperature approaches the Tm, a drop of the NPS signal is observed. The breakpoints in the temperatures are 22.5 °C, 41.0 °C, and 55.5 °C for DMPC, DPPC, and DSPC, respectively. These values are very close to the theoretical Tm values, i.e., 24 °C, 41.4 °C, and 55 °C for DMPC, DPPC, and DSPC, respectively. Our studies prove that the NPS methodology is a simple and valuable tool for the determination of the Tm of phospholipids.",
author = "Wen Chen and Filip Duša and Joanna Witos and Ruokonen, {Suvi Katriina} and Wiedmer, {Susanne K.}",
year = "2018",
month = "12",
day = "1",
doi = "10.1038/s41598-018-33107-5",
language = "English",
volume = "8",
journal = "Scientific Reports",
issn = "2045-2322",
number = "1",

}

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TY - JOUR

T1 - Determination of the Main Phase Transition Temperature of Phospholipids by Nanoplasmonic Sensing

AU - Chen, Wen

AU - Duša, Filip

AU - Witos, Joanna

AU - Ruokonen, Suvi Katriina

AU - Wiedmer, Susanne K.

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Our study demonstrates that nanoplasmonic sensing (NPS) can be utilized for the determination of the phase transition temperature (Tm) of phospholipids. During the phase transition, the lipid bilayer undergoes a conformational change. Therefore, it is presumed that the Tm of phospholipids can be determined by detecting conformational changes in liposomes. The studied lipids included 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC). Liposomes in gel phase are immobilized onto silicon dioxide sensors and the sensor cell temperature is increased until passing the Tm of the lipid. The results show that, when the system temperature approaches the Tm, a drop of the NPS signal is observed. The breakpoints in the temperatures are 22.5 °C, 41.0 °C, and 55.5 °C for DMPC, DPPC, and DSPC, respectively. These values are very close to the theoretical Tm values, i.e., 24 °C, 41.4 °C, and 55 °C for DMPC, DPPC, and DSPC, respectively. Our studies prove that the NPS methodology is a simple and valuable tool for the determination of the Tm of phospholipids.

AB - Our study demonstrates that nanoplasmonic sensing (NPS) can be utilized for the determination of the phase transition temperature (Tm) of phospholipids. During the phase transition, the lipid bilayer undergoes a conformational change. Therefore, it is presumed that the Tm of phospholipids can be determined by detecting conformational changes in liposomes. The studied lipids included 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC). Liposomes in gel phase are immobilized onto silicon dioxide sensors and the sensor cell temperature is increased until passing the Tm of the lipid. The results show that, when the system temperature approaches the Tm, a drop of the NPS signal is observed. The breakpoints in the temperatures are 22.5 °C, 41.0 °C, and 55.5 °C for DMPC, DPPC, and DSPC, respectively. These values are very close to the theoretical Tm values, i.e., 24 °C, 41.4 °C, and 55 °C for DMPC, DPPC, and DSPC, respectively. Our studies prove that the NPS methodology is a simple and valuable tool for the determination of the Tm of phospholipids.

UR - http://www.scopus.com/inward/record.url?scp=85054446384&partnerID=8YFLogxK

U2 - 10.1038/s41598-018-33107-5

DO - 10.1038/s41598-018-33107-5

M3 - Article

VL - 8

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 14815

ER -

ID: 28755925