Detection of raman scattering spectra of high spectral resolution in short oligonucleotides: Compared with the full-length DNA spectra

Research output: Contribution to journalArticleScientificpeer-review

Researchers

  • F. B. Bayramov
  • Vladimir V. Toporov
  • E.D. Poloskin
  • A.L. Chernev
  • Mikhail V. Dubina
  • Harri Lipsanen

  • Bakhysh H. Bairamov

Research units

  • RAS - Ioffe Physico Technical Institute
  • RAS - St. Petersburg Academic University

Abstract

The study focuses on examining the high spectral resolution spectra by high sensitivity nonresonant Raman scattering method in biomacromolecules on the example of singlestranded short oligonucleatides d(20G, 20T), where d is abbreviation from deoxyribonucleotide, G-guanine and T-thymine. Detection of the narrow spectral lines allows us to determine the characteristic time scale of vibrational excitation and makes it possible to study the dynamics of fast relaxation processes of atoms vibrational motions in biomacromolecules. Findings of the research show that for one of the narrowest line at 1355.4 sm-1, attributed to fluctuations of the methyl group of dT, the full width of the line on a half of its height equals 14.6 sm-1 and the appropriate life time equals 0.38 ps. We compared the obtained spectra with the complete Raman scattering spectra obtained from the dried-up fullsized DNA of a calf received with the high spectral and spatial resolution and observed in the broad spectral range of the frequency shifts of the scattered light from 6 to 4000 sm-1. The received results show that short oligonucleotides can be used as successful model objects for investigating the molecular DNA structure when studying their secondary structures. These data can be also required in creating and investigating different complexes with inorganic semiconductor nanostructures on the basis of such short oligonucleotides. The results obtained in the research make it possible to implement theoretical and experimental studies required for achieving a deeper fundamental understanding of structure-property relationship for quantum-confined semiconductors nanostructures and biomolecules and, are therefore of high interest to the new directions of rapidly developing nanobioelectronics.

Details

Original languageRussian
Pages (from-to)70-84
Number of pages15
JournalHerald of the Bauman Moscow State Technical University. Series Natural Sciences
Issue number2
Publication statusPublished - 2017
MoE publication typeA1 Journal article-refereed

    Research areas

  • DNA, Oligonucleotides, Spectroscopy of Raman scattering

ID: 13551054