Deposition of xylan isolated from Pennisetum purpureum on fibres of Eucalyptus globulus and characterisation of the composition of the surface xylans by immunolabelling and enzymatic peeling

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Deposition of xylan isolated from Pennisetum purpureum on fibres of Eucalyptus globulus and characterisation of the composition of the surface xylans by immunolabelling and enzymatic peeling. / Tamminen, Tarja; Mikkelson, Atte; Siika-Aho, Matti; Kruus, Kristiina; Pere, Jaakko; Borges Gomes, Fernando José; Colodette, Jorge Luiz.

In: Holzforschung, Vol. 72, No. 11, 01.11.2018, p. 915-922.

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Tamminen, Tarja ; Mikkelson, Atte ; Siika-Aho, Matti ; Kruus, Kristiina ; Pere, Jaakko ; Borges Gomes, Fernando José ; Colodette, Jorge Luiz. / Deposition of xylan isolated from Pennisetum purpureum on fibres of Eucalyptus globulus and characterisation of the composition of the surface xylans by immunolabelling and enzymatic peeling. In: Holzforschung. 2018 ; Vol. 72, No. 11. pp. 915-922.

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@article{0f231538c5ad43eb98c664f9fd22ef5c,
title = "Deposition of xylan isolated from Pennisetum purpureum on fibres of Eucalyptus globulus and characterisation of the composition of the surface xylans by immunolabelling and enzymatic peeling",
abstract = "Adsorption of xylan on pulp is a potential method to improve its properties, especially refinability for high quality printing and writing (P&W) paper grades. In this study, elephant grass [Pennisetum purpureum (Schumach.)] xylan was used for this purpose. The xylan was extracted using cold caustic extraction (CCE) from P. purpureum brown pulp, produced by the Soda-AQ process (kappa 20). Xylan resorption was accomplished during the oxygen delignification phase of eucalypt [Eucalyptus globulus (Labill.)] pulp to avoid problems induced by the colour of the lignin-contaminated deposited xylan. Immunolabelling and enzymatic peeling methodologies were compared for the analysis of the spatial distribution of xylan in the fibre wall. The labelling appeared unevenly as faint and brighter patches on fibre surfaces. Increased labelling of xylan was detected on the samples with precipitated P. purpureum xylan. The enzymatic peeling method using a total hydrolysis enzyme mixture yielded a composition gradient as a function of time, showing clear xylose (Xyl) enrichment in the very beginning of the reaction, reflecting hydrolysis of fibre surfaces. Pure xylanase and endoglucanase hydrolyses yielded different product patterns and kinetics compared to total hydrolysis, but interpretation of those results in terms of xylan localisation was not straightforward.",
keywords = "elephant grass, enzymatic hydrolysis, Eucalyptus globulus, fibre surface composition, immunolabelling, Pennisetum purpureum, xylan",
author = "Tarja Tamminen and Atte Mikkelson and Matti Siika-Aho and Kristiina Kruus and Jaakko Pere and {Borges Gomes}, {Fernando Jos{\'e}} and Colodette, {Jorge Luiz}",
year = "2018",
month = "11",
day = "1",
doi = "10.1515/hf-2018-0002",
language = "English",
volume = "72",
pages = "915--922",
journal = "Holzforschung",
issn = "0018-3830",
number = "11",

}

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TY - JOUR

T1 - Deposition of xylan isolated from Pennisetum purpureum on fibres of Eucalyptus globulus and characterisation of the composition of the surface xylans by immunolabelling and enzymatic peeling

AU - Tamminen, Tarja

AU - Mikkelson, Atte

AU - Siika-Aho, Matti

AU - Kruus, Kristiina

AU - Pere, Jaakko

AU - Borges Gomes, Fernando José

AU - Colodette, Jorge Luiz

PY - 2018/11/1

Y1 - 2018/11/1

N2 - Adsorption of xylan on pulp is a potential method to improve its properties, especially refinability for high quality printing and writing (P&W) paper grades. In this study, elephant grass [Pennisetum purpureum (Schumach.)] xylan was used for this purpose. The xylan was extracted using cold caustic extraction (CCE) from P. purpureum brown pulp, produced by the Soda-AQ process (kappa 20). Xylan resorption was accomplished during the oxygen delignification phase of eucalypt [Eucalyptus globulus (Labill.)] pulp to avoid problems induced by the colour of the lignin-contaminated deposited xylan. Immunolabelling and enzymatic peeling methodologies were compared for the analysis of the spatial distribution of xylan in the fibre wall. The labelling appeared unevenly as faint and brighter patches on fibre surfaces. Increased labelling of xylan was detected on the samples with precipitated P. purpureum xylan. The enzymatic peeling method using a total hydrolysis enzyme mixture yielded a composition gradient as a function of time, showing clear xylose (Xyl) enrichment in the very beginning of the reaction, reflecting hydrolysis of fibre surfaces. Pure xylanase and endoglucanase hydrolyses yielded different product patterns and kinetics compared to total hydrolysis, but interpretation of those results in terms of xylan localisation was not straightforward.

AB - Adsorption of xylan on pulp is a potential method to improve its properties, especially refinability for high quality printing and writing (P&W) paper grades. In this study, elephant grass [Pennisetum purpureum (Schumach.)] xylan was used for this purpose. The xylan was extracted using cold caustic extraction (CCE) from P. purpureum brown pulp, produced by the Soda-AQ process (kappa 20). Xylan resorption was accomplished during the oxygen delignification phase of eucalypt [Eucalyptus globulus (Labill.)] pulp to avoid problems induced by the colour of the lignin-contaminated deposited xylan. Immunolabelling and enzymatic peeling methodologies were compared for the analysis of the spatial distribution of xylan in the fibre wall. The labelling appeared unevenly as faint and brighter patches on fibre surfaces. Increased labelling of xylan was detected on the samples with precipitated P. purpureum xylan. The enzymatic peeling method using a total hydrolysis enzyme mixture yielded a composition gradient as a function of time, showing clear xylose (Xyl) enrichment in the very beginning of the reaction, reflecting hydrolysis of fibre surfaces. Pure xylanase and endoglucanase hydrolyses yielded different product patterns and kinetics compared to total hydrolysis, but interpretation of those results in terms of xylan localisation was not straightforward.

KW - elephant grass

KW - enzymatic hydrolysis

KW - Eucalyptus globulus

KW - fibre surface composition

KW - immunolabelling

KW - Pennisetum purpureum

KW - xylan

UR - http://www.scopus.com/inward/record.url?scp=85053530976&partnerID=8YFLogxK

U2 - 10.1515/hf-2018-0002

DO - 10.1515/hf-2018-0002

M3 - Article

VL - 72

SP - 915

EP - 922

JO - Holzforschung

JF - Holzforschung

SN - 0018-3830

IS - 11

ER -

ID: 32864252