Delivery of agents into articular cartilage with electric spark-induced sound waves

Alejandro García Pérez, Heikki J. Nieminen*, Mikko Finnilä, Ari Salmi, Kenneth P.H. Pritzker, Eetu Lampsijärvi, Tor Paulin, Anu J. Airaksinen, Simo Saarakkala, Edward Hæggström

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

1 Citation (Scopus)
158 Downloads (Pure)

Abstract

Localized delivery of drugs into articular cartilage (AC) may facilitate the development of novel therapies to treat osteoarthritis (OA). We investigated the potential of spark-gap-generated sound to deliver a drug surrogate, i.e., methylene blue (MB), into AC. In vitro experiments exposed bovine AC samples to either simultaneous sonication and immersion in MB (Treatment 1; n = 10), immersion in MB after sonication (Control 1; n = 10), solely immersion in MB (Control 2; n = 10), or neither sonication nor immersion in MB (Control 3; n = 10). The sonication protocol consisted of 1,000 spark-gap -generated pulses. Delivery of MB into AC was estimated from optical absorbance in transmission light microscopy. Optical absorbance was significantly greater in the treatment group up to 900 μm depth from AC surface as compared to all controls. Field emission scanning electron microscopy (FESEM), histological analysis, and digital densitometry (DD) of sonicated (n = 6) and non-sonicated (n = 6) samples showed no evidence of sonication-induced changes in proteoglycan content or collagen structure. Consequently, spark-gap -generated sound may offer a solution for localized drug delivery into AC in a non-destructive fashion. Further research on this method may contribute to OA drug therapies.

Original languageEnglish
Article number116
Pages (from-to)1-7
JournalFrontiers in Physics
Volume6
Issue numberOCT
DOIs
Publication statusPublished - 16 Oct 2018
MoE publication typeA1 Journal article-refereed

Keywords

  • Cartilage
  • Drug delivery
  • Electric sparks
  • Osteoarthritis therapies
  • Sound
  • Ultrasound

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