Methanotrophs closely related to psychrotolerant members of the genera Methylobacter and Methylocella were identified in cultures enriched at 10A degrees C from landfill cover soil samples collected in the period from April to November. Mesophilic methanotrophs of the genera Methylobacter and Methylosinus were found in cultures enriched at 20A degrees C from the same cover soil samples. A thermotolerant methanotroph related to Methylocaldum gracile was identified in the culture enriched at 40A degrees C from a sample collected in May (the temperature of the cover soil was 11.5-12.5A degrees C). In addition to methanotrophs, methylobacteria of the genera Methylotenera and Methylovorus and members of the genera Verrucomicrobium, Pseudomonas, Pseudoxanthomonas, Dokdonella, Candidatus Protochlamydia, and Thiorhodospira were also identified in the enrichment cultures. A methanotroph closely related to the psychrotolerant species Methylobacter tundripaludum (98% sequence identity of 16S rRNA genes with the type strain SV96(T)) was isolated in pure culture. The introduction of a mixture of the methanotrophic enrichments, grown at 15A degrees C, into the landfill cover soil resulted in a decrease in methane emission from the landfill surface in autumn (October, November). The inoculum used was demonstrated to contain methanotrophs closely related to Methylobacter tundripaludum SV96.
- aerobic methanotrophic bacteria (methanotrophs)
- psychrotolerant methanotrophs
- landfills for disposal of municipal solid waste (MSW)
- denaturing gradient gel electrophoresis (DGGE)
- 16S RIBOSOMAL-RNA
- SP NOV.
- DIAGNOSTIC MICROARRAY
- PHYLOGENETIC ANALYSIS
- ACTIVE METHANOTROPHS
- GEN. NOV.