Crystallization and preliminary X-ray analysis of a xyloglucan endotransglycosylase from Populus tremula x tremuloides

P Johansson*, S Denman, H Brumer, AM Kallas, H Henriksson, T Bergfors, TT Teeri, TA Jones

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls. Recombinant XET from poplar has been purified from a Pichia pastoris expression system and crystallized. Two different crystal forms were obtained by vapour diffusion from potassium sodium tartrate and from an imidazole buffer using sodium acetate as a precipitant. Data were collected from these crystal forms to 3.5 and 2.1 Angstrom resolution, respectively. The first crystal form was found to belong to space group P3(1)21 or P3(2)21 (unit-cell parameters a = 98.6, b = 98.6, c = 98.5 Angstrom) and the second crystal form to space group P6(3) (unit-cell parameters a = 188.7, b = 188.7, c = 46.1 Angstrom).

Original languageEnglish
Pages (from-to)535-537
Number of pages3
JournalACTA CRYSTALLOGRAPHICA SECTION D : BIOLOGICAL CRYSTALLOGRAPHY
Volume59
Publication statusPublished - Mar 2003
MoE publication typeA1 Journal article-refereed

Keywords

  • CRYSTAL-STRUCTURE
  • ARABIDOPSIS TCH4
  • WALL

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