Crystallization and preliminary X-ray analysis of a xyloglucan endotransglycosylase from Populus tremula x tremuloides

P Johansson; S Denman; H Brumer; AM Kallas; H Henriksson; T Bergfors; TT Teeri; TA Jones

Crystallization and preliminary X-ray analysis of a xyloglucan endotransglycosylase from Populus tremula x tremuloides

P Johansson^*, S Denman, H Brumer, AM Kallas, H Henriksson, T Bergfors, TT Teeri, TA Jones

^*Corresponding author for this work

Not published at Aalto University

Research output: Contribution to journal › Article › Scientific › peer-review

Abstract

Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls. Recombinant XET from poplar has been purified from a Pichia pastoris expression system and crystallized. Two different crystal forms were obtained by vapour diffusion from potassium sodium tartrate and from an imidazole buffer using sodium acetate as a precipitant. Data were collected from these crystal forms to 3.5 and 2.1 Angstrom resolution, respectively. The first crystal form was found to belong to space group P3(1)21 or P3(2)21 (unit-cell parameters a = 98.6, b = 98.6, c = 98.5 Angstrom) and the second crystal form to space group P6(3) (unit-cell parameters a = 188.7, b = 188.7, c = 46.1 Angstrom).

Original language	English
Pages (from-to)	535-537
Number of pages	3
Journal	Acta Crystallographica. Section D: Biological Crystallography
Volume	59
Publication status	Published - Mar 2003
MoE publication type	A1 Journal article-refereed

Keywords

CRYSTAL-STRUCTURE
ARABIDOPSIS TCH4
WALL

Cite this

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title = "Crystallization and preliminary X-ray analysis of a xyloglucan endotransglycosylase from Populus tremula x tremuloides",

abstract = "Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls. Recombinant XET from poplar has been purified from a Pichia pastoris expression system and crystallized. Two different crystal forms were obtained by vapour diffusion from potassium sodium tartrate and from an imidazole buffer using sodium acetate as a precipitant. Data were collected from these crystal forms to 3.5 and 2.1 Angstrom resolution, respectively. The first crystal form was found to belong to space group P3(1)21 or P3(2)21 (unit-cell parameters a = 98.6, b = 98.6, c = 98.5 Angstrom) and the second crystal form to space group P6(3) (unit-cell parameters a = 188.7, b = 188.7, c = 46.1 Angstrom).",

keywords = "CRYSTAL-STRUCTURE, ARABIDOPSIS TCH4, WALL",

author = "P Johansson and S Denman and H Brumer and AM Kallas and H Henriksson and T Bergfors and TT Teeri and TA Jones",

year = "2003",

month = mar,

language = "English",

volume = "59",

pages = "535--537",

journal = "Acta Crystallographica. Section D: Biological Crystallography",

issn = "0907-4449",

publisher = "Wiley-Blackwell",

}

TY - JOUR

T1 - Crystallization and preliminary X-ray analysis of a xyloglucan endotransglycosylase from Populus tremula x tremuloides

AU - Johansson, P

AU - Denman, S

AU - Brumer, H

AU - Kallas, AM

AU - Henriksson, H

AU - Bergfors, T

AU - Teeri, TT

AU - Jones, TA

PY - 2003/3

Y1 - 2003/3

N2 - Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls. Recombinant XET from poplar has been purified from a Pichia pastoris expression system and crystallized. Two different crystal forms were obtained by vapour diffusion from potassium sodium tartrate and from an imidazole buffer using sodium acetate as a precipitant. Data were collected from these crystal forms to 3.5 and 2.1 Angstrom resolution, respectively. The first crystal form was found to belong to space group P3(1)21 or P3(2)21 (unit-cell parameters a = 98.6, b = 98.6, c = 98.5 Angstrom) and the second crystal form to space group P6(3) (unit-cell parameters a = 188.7, b = 188.7, c = 46.1 Angstrom).

AB - Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls. Recombinant XET from poplar has been purified from a Pichia pastoris expression system and crystallized. Two different crystal forms were obtained by vapour diffusion from potassium sodium tartrate and from an imidazole buffer using sodium acetate as a precipitant. Data were collected from these crystal forms to 3.5 and 2.1 Angstrom resolution, respectively. The first crystal form was found to belong to space group P3(1)21 or P3(2)21 (unit-cell parameters a = 98.6, b = 98.6, c = 98.5 Angstrom) and the second crystal form to space group P6(3) (unit-cell parameters a = 188.7, b = 188.7, c = 46.1 Angstrom).

KW - CRYSTAL-STRUCTURE

KW - ARABIDOPSIS TCH4

KW - WALL

M3 - Article

SN - 0907-4449

VL - 59

SP - 535

EP - 537

JO - Acta Crystallographica. Section D: Biological Crystallography

JF - Acta Crystallographica. Section D: Biological Crystallography

ER -

Crystallization and preliminary X-ray analysis of a xyloglucan endotransglycosylase from Populus tremula x tremuloides

Abstract

Keywords

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