Controlled communication between physically separated bacterial populations in a microfluidic device

Research output: Contribution to journalArticle

Standard

Harvard

APA

Vancouver

Author

Bibtex - Download

@article{736d7c61b2ea44a99accda327014f932,
title = "Controlled communication between physically separated bacterial populations in a microfluidic device",
abstract = "The engineering of microbial systems increasingly strives to achieve a co-existence and co-functioning of different populations. By creating interactions, one can utilize combinations of cells where each population has a specialized function, such as regulation or sharing of metabolic burden. Here we describe a microfluidic system that enables long-term and independent growth of fixed and distinctly separate microbial populations, while allowing communication through a thin nano-cellulose filter. Using quorum-sensing signaling, we can couple the populations and show that this leads to a rapid and stable connection over long periods of time. We continue to show that this control over communication can be utilized to drive nonlinear responses. The coupling of separate populations, standardized interaction, and context-independent function lay the foundation for the construction of increasingly complex community-wide dynamic genetic regulatory mechanisms.",
author = "Ekaterina Osmekhina and Christopher Jonkergouw and Georg Schmidt and Farzin Jahangiri and Ville Jokinen and Sami Franssila and Markus Linder",
year = "2018",
doi = "10.1038/s42003-018-0102-y",
language = "English",
volume = "1",
journal = "Communications Biology",
issn = "2399-3642",
publisher = "Nature Publishing Group",

}

RIS - Download

TY - JOUR

T1 - Controlled communication between physically separated bacterial populations in a microfluidic device

AU - Osmekhina, Ekaterina

AU - Jonkergouw, Christopher

AU - Schmidt, Georg

AU - Jahangiri, Farzin

AU - Jokinen, Ville

AU - Franssila, Sami

AU - Linder, Markus

PY - 2018

Y1 - 2018

N2 - The engineering of microbial systems increasingly strives to achieve a co-existence and co-functioning of different populations. By creating interactions, one can utilize combinations of cells where each population has a specialized function, such as regulation or sharing of metabolic burden. Here we describe a microfluidic system that enables long-term and independent growth of fixed and distinctly separate microbial populations, while allowing communication through a thin nano-cellulose filter. Using quorum-sensing signaling, we can couple the populations and show that this leads to a rapid and stable connection over long periods of time. We continue to show that this control over communication can be utilized to drive nonlinear responses. The coupling of separate populations, standardized interaction, and context-independent function lay the foundation for the construction of increasingly complex community-wide dynamic genetic regulatory mechanisms.

AB - The engineering of microbial systems increasingly strives to achieve a co-existence and co-functioning of different populations. By creating interactions, one can utilize combinations of cells where each population has a specialized function, such as regulation or sharing of metabolic burden. Here we describe a microfluidic system that enables long-term and independent growth of fixed and distinctly separate microbial populations, while allowing communication through a thin nano-cellulose filter. Using quorum-sensing signaling, we can couple the populations and show that this leads to a rapid and stable connection over long periods of time. We continue to show that this control over communication can be utilized to drive nonlinear responses. The coupling of separate populations, standardized interaction, and context-independent function lay the foundation for the construction of increasingly complex community-wide dynamic genetic regulatory mechanisms.

U2 - 10.1038/s42003-018-0102-y

DO - 10.1038/s42003-018-0102-y

M3 - Article

VL - 1

JO - Communications Biology

JF - Communications Biology

SN - 2399-3642

M1 - 97

ER -

ID: 30429613