Control of Protein Affinity of Bioactive Nanocellulose and Passivation Using Engineered Block and Random Copolymers

Research output: Contribution to journalArticleScientificpeer-review

Researchers

Research units

  • VTT Technical Research Centre of Finland
  • DWI-Leibniz-Institute for Interactive Materials

Abstract

We passivated TEMPO-oxidized cellulose nanofibrils (TOCNF) toward human immunoglobulin G (hIgG) by modification with block and random copolymers of poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) and poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA). The block copolymers reversibly adsorbed on TOCNF and were highly effective in preventing nonspecific interactions with hIgG, especially if short PDMAEMA blocks were used. In such cases, total protein rejection was achieved. This is in contrast to typical blocking agents, which performed poorly. When an anti-human IgG biointerface was installed onto the passivated TOCNF, remarkably high affinity antibody-antigen interactions were observed (0.90 ± 0.09 mg/m2). This is in contrast to the nonpassivated biointerface, which resulted in a significant false response. In addition, regeneration of the biointerface was possible by low pH aqueous wash. Protein A from Staphylococcus aureus was also utilized to successfully increase the sensitivity for human IgG recognition (1.28 ± 0.11 mg/m2). Overall, the developed system based on TOCNF modified with multifunctional polymers can be easily deployed as bioactive material with minimum fouling and excellent selectivity.

Details

Original languageEnglish
Pages (from-to)5668-5678
Number of pages11
JournalACS Applied Materials and Interfaces
Volume8
Issue number8
Publication statusPublished - 2016
MoE publication typeA1 Journal article-refereed

    Research areas

  • antifouling, biosurfaces, cellulose nanofibrils, human IgG, nonspecific adsorption, PDMAEMA, POEGMA, TEMPO-oxidation

ID: 1800875