CONSTRUCTION AND CHARACTERIZATION OF CELLULOLYTIC YEASTS.

Merja Penttila*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingOther chapter contributionpeer-review

2 Citations (Scopus)

Abstract

The successful construction of cellulolytic yeast strains could lead to improvements in the traditional processes, such as brewing, distilling and wine making, and to more versatile utilization of cellulose for energy production and in novel yeast based applications. Five different cellulolytic yeast strains were constructed in this study. The genes coding for the cellobiohydrolases CBHI and CBHII, and the endoglucanases EGI and EGIII of the filamentous fungus Trichoderma reesei, as well as the beta -glucosidase gene of Aspergillus niger, were expressed in the yeast S. cerevisiae. All recombinant yeast strains were able to hydrolyse natural cellulosic substrates present in yeast culture medium showing different cellulolytic capabilities.

Original languageEnglish
Title of host publicationPublications - Technical Research Centre of Finland
Pages1-53
Number of pages53
Edition39
Publication statusPublished - May 1987

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