Computer-aided production of scaffolded DNA nanostructures from flat sheet meshes

Erik Benson, Abdulmelik Mohammed, Alessandro Bosco, Ana I. Teixeira, Pekka Orponen, Björn Högberg*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

26 Citations (Scopus)
167 Downloads (Pure)

Abstract

The use of DNA as a nanoscale construction material has been a rapidly developing field since the 1980s, in particular since the introduction of scaffolded DNA origami in 2006. Although software is available for DNA origami design, the user is generally limited to architectures where finding the scaffold path through the object is trivial. Herein, we demonstrate the automated conversion of arbitrary two-dimensional sheets in the form of digital meshes into scaffolded DNA nanostructures. We investigate the properties of DNA meshes based on three different internal frameworks in standard folding buffer and physiological salt buffers. We then employ the triangulated internal framework and produce four 2D structures with complex outlines and internal features. We demonstrate that this highly automated technique is capable of producing complex DNA nanostructures that fold with high yield to their programmed configurations, covering around 70 % more surface area than classic origami flat sheets.

Original languageEnglish
Pages (from-to)8869-8872
Number of pages4
JournalAngewandte Chemie
Volume55
Issue number31
DOIs
Publication statusPublished - 2016
MoE publication typeA1 Journal article-refereed

Keywords

  • atomic force microscopy
  • DNA origami
  • DNA structures
  • molecular simulation

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