Comparative genomic and functional analysis of Lactobacillus casei and Lactobacillus rhamnosus strains marketed as probiotics

Francois P. Douillard, Angela Ribbera, Hanna M. Järvinen, Ravi Kant, Taija E. Pietilä, Cinzia Randazzo, Lars Paulin, Pia K. Laine, Cinzia Caggia, Ingemar von Ossowski, Justus Reunanen, Reetta Satokari, Seppo Salminen, Airi Palva, Willem M. de Vos*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Four Lactobacillus strains were isolated from marketed probiotic products, including L. rhamnosus strains from Vifit (Friesland Campina) and Idoform (Ferrosan) and L. casei strains from Actimel (Danone) and Yakult (Yakult Honsa Co.). Their genomes and phenotypes were characterized and compared in detail with L. casei strain BL23 and L. rhamnosus strain GG. Phenotypic analysis of the new isolates indicated differences in carbohydrate utilization between L. casei and L. rhamnosus strains, which could be linked to their genotypes. The two isolated L. rhamnosus strains had genomes that were virtually identical to that of L. rhamnosus GG, testifying to their genomic stability and integrity in food products. The L. casei strains showed much greater genomic heterogeneity. Remarkably, all strains contained an intact spaCBA pilus gene cluster. However, only the L. rhamnosus strains produced mucus-binding SpaCBA pili under the conditions tested. Transcription initiation mapping demonstrated that the insertion of an iso-IS30 element upstream of the pilus gene cluster in L. rhamnosus strains but absent in L. casei strains had constituted a functional promoter driving pilus gene expression. All L. rhamnosus strains triggered an NF-kappa B response via Toll-like receptor 2 (TLR2) in a reporter cell line, whereas the L. casei strains did not or did so to a much lesser extent. This study demonstrates that the two L. rhamnosus strains isolated from probiotic products are virtually identical to L. rhamnosus GG and further highlights the differences between these and L. casei strains widely marketed as probiotics, in terms of genome content, mucus-binding and metabolic capacities, and host signaling capabilities.

Original languageEnglish
Pages (from-to)1923-1933
Number of pages11
JournalApplied and Environmental Microbiology
Volume79
Issue number6
DOIs
Publication statusPublished - Mar 2013
MoE publication typeA1 Journal article-refereed

Keywords

  • LACTIC-ACID BACTERIA
  • BINDING-PROTEIN
  • IN-VITRO
  • CARBOHYDRATE-METABOLISM
  • SEQUENCE
  • GG
  • ADHESION
  • BL23
  • STRESS
  • MUCUS

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