Comparative analysis of the bacterial diversity in a lab-scale moving bed biofilm reactor (MBBR) applied to treat urban wastewater under different operational conditions

Research output: Contribution to journalArticleScientificpeer-review


  • Kadiya Calderón
  • Jaime Martín-Pascual
  • José Manuel Poyatos
  • Belén Rodelas
  • Alejandro González-Martínez
  • Jesús González-López

Research units

  • University of Granada


Different types of carriers were tested as support material in a lab-scale moving bed biofilm reactor (MBBR) used to treat urban wastewater under three different conditions of hydraulic retention time (HRT) and carrier filling ratios (FR). The bacterial diversity developed on the biofilms responsible of the treatment was studied using a cultivation-independent approach based on the polymerase chain reaction-temperature gradient gel electrophoresis technique (PCR-TGGE). Cluster analysis of TGGE fingerprints showed significant differences of community structure dependent upon the different operational conditions applied. Redundancy analysis (RDA) was used to determine the relationship between the operational conditions (type of carrier, HRT, FR) and bacterial biofilm diversity, demonstrating a significant effect of FR = 50%. Phylogenetic analysis of PCR-reamplified and sequenced TGGE bands revealed that the prevalent Bacteria populations in the biofilm were related to Betaproteobacteria (46%), Firmicutes (34%),Alphaproteobacteria (14%) and Gammaproteobacteria (9%).


Original languageEnglish
Pages (from-to)119-126
Number of pages8
JournalBioresource Technology
Publication statusPublished - Oct 2012
MoE publication typeA1 Journal article-refereed

    Research areas

  • Biofilm, Carrier-FR, HRT, MBBR, TGGE

ID: 8886355