Comparative analysis of human and mouse transcriptomes of Th17 cell priming

Research output: Contribution to journalArticle

Researchers

  • Soile Tuomela
  • Sini Rautio

  • Helena Ahlfors
  • Viveka Öling
  • Verna Salo
  • Ubaid Ullah
  • Zhi Chen
  • Saara Hämälistö
  • Subhash K. Tripathi
  • Tarmo Äijö
  • Omid Rasool
  • Hayssam Soueidan
  • Lodewyk Wessels
  • Brigitta Stockinger
  • Harri Lähdesmäki

  • Riitta Lahesmaa

Research units

  • Turku Centre for Biotechnology
  • Medical Research Council
  • Netherlands Cancer Institute

Abstract

Uncontrolled Th17 cell activity is associated with cancer and autoimmune and inflammatory diseases. To validate the potential relevance of mouse models of targeting the Th17 pathway in human diseases we used RNA sequencing to compare the expression of coding and non-coding transcripts during the priming of Th17 cell differentiation in both human and mouse. In addition to already known targets, several transcripts not previously linked to Th17 cell polarization were found in both species. Moreover, a considerable number of human-specific long non-coding RNAs were identified that responded to cytokines stimulating Th17 cell differentiation. We integrated our transcriptomics data with known disease-associated polymorphisms and show that conserved regulation pinpoints genes that are relevant to Th17 cell-mediated human diseases and that can be modelled in mouse. Substantial differences observed in non-coding transcriptomes between the two species as well as increased overlap between Th17 cell-specific gene expression and disease-associated polymorphisms underline the need of parallel analysis of human and mouse models. Comprehensive analysis of genes regulated during Th17 cell priming and their classification to conserved and non-conserved between human and mouse facilitates translational research, pointing out which candidate targets identified in human are worth studying by using in vivo mouse models.

Details

Original languageEnglish
Pages (from-to)13416-13428
Number of pages13
JournalONCOTARGET
Volume7
Issue number12
Publication statusPublished - 22 Mar 2016
MoE publication typeA1 Journal article-refereed

    Research areas

  • Comparative analysis of human and mouse, Disease-associated SNPs, Immune response, Immunity, Immunology and microbiology section, LncRNA, RNA-seq, Th17 cell priming

ID: 4810135