Coacervation of resilin fusion proteins containing terminal functionalities

Research output: Contribution to journalArticleScientificpeer-review

Details

Original languageEnglish
Pages (from-to)590-596
Number of pages7
JournalColloids and Surfaces B: Biointerfaces
Volume171
Publication statusPublished - 1 Nov 2018
MoE publication typeA1 Journal article-refereed

Researchers

Research units

  • VTT Technical Research Centre of Finland

Abstract

Liquid-liquid phase transition known as coacervation of resilin-like-peptide fusion proteins containing different terminal domains were investigated. Two different modular proteins were designed and produced and their behavior were compared to a resilin-like-peptide without terminal domains. The size of the particle-like coacervates was modulated by the protein concentration, pH and temperature. The morphology and three-dimensional (3D) structural details of the coacervate particles were investigated by cryogenic transmission electron microscopy (cryo-TEM) and tomography (cryo-ET) reconstruction. Selective adhesion of the coacervates on cellulose and graphene surfaces was demonstrated.

    Research areas

  • Coacervation, Resilin, Selective adhesion, Self-assembly, Tomography

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