Projects per year
Abstract
Liquid-liquid phase transition known as coacervation of resilin-like-peptide fusion proteins containing different terminal domains were investigated. Two different modular proteins were designed and produced and their behavior were compared to a resilin-like-peptide without terminal domains. The size of the particle-like coacervates was modulated by the protein concentration, pH and temperature. The morphology and three-dimensional (3D) structural details of the coacervate particles were investigated by cryogenic transmission electron microscopy (cryo-TEM) and tomography (cryo-ET) reconstruction. Selective adhesion of the coacervates on cellulose and graphene surfaces was demonstrated.
Original language | English |
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Pages (from-to) | 590-596 |
Number of pages | 7 |
Journal | Colloids and Surfaces B: Biointerfaces |
Volume | 171 |
DOIs | |
Publication status | Published - 1 Nov 2018 |
MoE publication type | A1 Journal article-refereed |
Keywords
- Resilin
- Self-assembly
- Coacervation
- Selective adhesion
- Tomography
- TROPOELASTIN
- HYDROPHOBINS
- CELLULOSE
- REC1-RESILIN
- GRAPHENE
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Dive into the research topics of 'Coacervation of resilin fusion proteins containing terminal functionalities'. Together they form a unique fingerprint.Projects
- 1 Finished
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HYBER: The Academy of Finland's Centre of Excellence in Molecular Engineering of Biosynthetic Hybrid Materials research (2014-2019)
Ikkala, O. (Principal investigator), Morits, M. (Project Member), Rissanen, S. (Project Member), Nonappa, N. (Project Member), Sanchez Sanchez, A. (Project Member), Bertula, K. (Project Member), Cherian, T. (Project Member), Haataja, J. (Project Member), Toivonen, M. (Project Member), Poutanen, M. (Project Member), Sohrabi, F. (Project Member), Hynninen, V. (Project Member) & Myllymäki, T. (Project Member)
01/01/2017 → 31/12/2019
Project: Academy of Finland: Other research funding
Equipment
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Bioeconomy Research Infrastructure
Seppälä, J. (Manager)
School of Chemical EngineeringFacility/equipment: Facility
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OtaNano - Nanomicroscopy Center
Seitsonen, J. (Manager) & Rissanen, A. (Other)
OtaNanoFacility/equipment: Facility