Cloning of Two Genes (LAT1,2) Encoding Specific L-Arabinose Transporters of the L-Arabinose Fermenting Yeast Ambrosiozyma monospora

Ritva Verho, Merja Penttila, Peter Richard*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

Abstract

We identified and characterized two genes, LAT1 and LAT2, which encode specific L-arabinose transporters. The genes were identified in the L-arabinose fermenting yeast Ambrosiozyma monospora. The yeast Saccharomyces cerevisiae had only very low L-arabinose transport activity; however, when LAT1 or LAT2 was expressed, L-arabinose transport was facilitated. When the LAT1 or LAT2 were expressed in an S. cerevisiae mutant where the main hexose transporters were deleted, the L-arabinose transporters could not restore growth on D-glucose, D-fructose, D-mannose or D-galactose. This indicates that these sugars are not transported and suggests that the transporters are specific for L-arabinose.

Original languageEnglish
Pages (from-to)604-611
Number of pages8
JournalApplied Biochemistry and Biotechnology
Volume164
Issue number5
DOIs
Publication statusPublished - Jul 2011
MoE publication typeA1 Journal article-refereed

Keywords

  • Sugar transport
  • L-Arabinose transport
  • L-Arabinose metabolism
  • Pentose fermentation
  • Metabolic engineering
  • Yeast
  • L-XYLULOSE REDUCTASE
  • SACCHAROMYCES-CEREVISIAE
  • ASPERGILLUS-NIGER
  • HEXOSE TRANSPORTERS
  • CATABOLIC PATHWAY
  • METABOLISM
  • GLUCOSE
  • IDENTIFICATION
  • EXPRESSION
  • ETHANOL

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