CHARACTERIZATION OF THE GENES OF THE 2,3-BUTANEDIOL OPERONS FROM KLEBSIELLA-TERRIGENA AND ENTEROBACTER-AEROGENES

K BLOMQVIST, M NIKKOLA, P LEHTOVAARA, ML SUIHKO, U AIRAKSINEN, KB STRABY, JKC KNOWLES, Merja Penttilä

Research output: Contribution to journalArticleScientificpeer-review

Abstract

The genes involved in the 2,3-butanediol pathway coding for alpha-acetolactate decarboxylase, alpha-acetolactate synthase (alpha-ALS), and acetoin (diacetyl) reductase were isolated from Klebsiella terrigena and shown to be located in one operon. This operon was also shown to exist in Enterobacter aerogenes. The budA gene, coding for alpha-acetolactate decarboxylase, gives in both organisms a protein of 259 amino acids. The amino acid similarity between these proteins is 87%. The K. terrigena genes budB and budC, coding for alpha-ALS and acetoin reductase, respectively, were sequenced. The 559-amino-acid-long alpha-ALS enzyme shows similarities to the large subunits of the Escherichia coli anabolic alpha-ALS enzymes encoded by the genes ilvB, ilvG, and ilvI. The K. terrigena alpha-ALS is also shown to complement an anabolic alpha-ALS-deficient E. coli strain for valine synthesis. The 243-amino-acid-long acetoin reductase has the consensus amino acid sequence for the insect-type alcohol dehydrogenase/ribitol dehydrogenase family and has extensive similarities with the N-terminal and internal regions of three known dehydrogenases and one oxidoreductase.

Original languageEnglish
Pages (from-to)1392-1404
Number of pages13
JournalJournal of Bacteriology
Volume175
Issue number5
Publication statusPublished - Mar 1993
MoE publication typeA1 Journal article-refereed

Keywords

  • ALPHA-ACETOLACTATE DECARBOXYLASE
  • ESCHERICHIA-COLI K-12
  • BREWERS-YEAST
  • EXPRESSION
  • PURIFICATION
  • CLONING
  • DEHYDROGENASE
  • SEQUENCE

Cite this

BLOMQVIST, K., NIKKOLA, M., LEHTOVAARA, P., SUIHKO, ML., AIRAKSINEN, U., STRABY, KB., KNOWLES, JKC., & Penttilä, M. (1993). CHARACTERIZATION OF THE GENES OF THE 2,3-BUTANEDIOL OPERONS FROM KLEBSIELLA-TERRIGENA AND ENTEROBACTER-AEROGENES. Journal of Bacteriology, 175(5), 1392-1404.