Projects per year
Abstract
Background: The Carbohydrate-Active enZymes (CAZy) auxiliary activity family 3 (AA3) comprises flavin adenine dinucleotide-dependent (FAD) oxidoreductases from the glucose–methanol–choline (GMC) family, which play auxiliary roles in lignocellulose conversion. The AA3 subfamily 1 predominantly consists of cellobiose dehydrogenases (CDHs) that typically comprise a dehydrogenase domain, a cytochrome domain, and a carbohydrate-binding module from family 1 (CBM1). Results: In this work, an AA3_1 gene from T. myriococcoides CBS 398.93 encoding only a GMC dehydrogenase domain was expressed in Aspergillus niger. Like previously characterized CDHs, this enzyme (TmXdhA) predominantly accepts linear saccharides with β-(1 → 4) linkage and targets the hydroxyl on the reducing anomeric carbon. TmXdhA was distinguished, however, by its preferential activity towards xylooligosaccharides over cellooligosaccharides. Amino acid sequence analysis showed that TmXdhA possesses a glutamine at the substrate-binding site rather than a threonine or serine that occupies this position in previously characterized CDHs, and structural models suggest the glutamine in TmXdhA could facilitate binding to pentose sugars. Conclusions: The biochemical analysis of TmXdhA revealed a catalytic preference for xylooligosaccharide substrates. The modeled structure of TmXdhA provides a reference for the screening of oxidoreductases targeting xylooligosaccharides. We anticipate TmXdhA to be a good candidate for the conversion of xylooligosaccharides to added-value chemicals by its exceptional catalytic ability.
Original language | English |
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Article number | 135 |
Number of pages | 17 |
Journal | Biotechnology for Biofuels and Bioproducts |
Volume | 15 |
Issue number | 1 |
DOIs | |
Publication status | Published - 7 Dec 2022 |
MoE publication type | A1 Journal article-refereed |
Keywords
- AA3_1
- CAZy AA3
- Cellobiose dehydrogenase
- Thermothelomyces myriococcoides
- Xylooligosaccharide dehydrogenase
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Dive into the research topics of 'Characterization of a novel AA3_1 xylooligosaccharide dehydrogenase from Thermothelomyces myriococcoides CBS 398.93'. Together they form a unique fingerprint.Datasets
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Characterization of a novel AA3_1 xylooligosaccharide dehydrogenase from Thermothelomyces myriococcoides CBS 398.93
Zhao, H. (Creator), Karppi, J. (Creator), Nguyen, T. T. M. (Creator), Bellemare, A. (Creator), Tsang, A. (Creator), Master, E. (Creator) & Tenkanen, M. (Creator), figshare, 13 Apr 2023
DOI: 10.6084/m9.figshare.c.6583855, https://springernature.figshare.com/collections/Characterization_of_a_novel_AA3_1_xylooligosaccharide_dehydrogenase_from_Thermothelomyces_myriococcoides_CBS_398_93/6583855
Dataset
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Additional file 1 of Characterization of a novel AA3_1 xylooligosaccharide dehydrogenase from Thermothelomyces myriococcoides CBS 398.93
Zhao, H. (Creator), Karppi, J. (Creator), Nguyen, T. T. M. (Creator), Bellemare, A. (Creator), Tsang, A. (Creator), Master, E. (Creator) & Tenkanen, M. (Creator), figshare, 13 Apr 2023
DOI: 10.6084/m9.figshare.22609450, https://springernature.figshare.com/articles/dataset/Additional_file_1_of_Characterization_of_a_novel_AA3_1_xylooligosaccharide_dehydrogenase_from_Thermothelomyces_myriococcoides_CBS_398_93/22609450
Dataset
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Additional file 3 of Characterization of a novel AA3_1 xylooligosaccharide dehydrogenase from Thermothelomyces myriococcoides CBS 398.93
Zhao, H. (Creator), Karppi, J. (Creator), Nguyen, T. T. M. (Creator), Bellemare, A. (Creator), Tsang, A. (Creator), Master, E. (Creator) & Tenkanen, M. (Creator), figshare, 13 Apr 2023
DOI: 10.6084/m9.figshare.22609456.v1, https://springernature.figshare.com/articles/dataset/Additional_file_3_of_Characterization_of_a_novel_AA3_1_xylooligosaccharide_dehydrogenase_from_Thermothelomyces_myriococcoides_CBS_398_93/22609456/1
Dataset
Projects
- 2 Finished
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BIOSEMBL: Transaminases for BIOcatalytic cascades for hemicellulose reasSEMBLy
Master, E. (Principal investigator)
31/10/2018 → 30/04/2022
Project: Other external funding: Other foreign funding
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Capturing Oxidoreductases for Carbohydrate Oligomer Activation
Master, E. (Principal investigator)
01/09/2017 → 31/12/2020
Project: Academy of Finland: Other research funding