Projects per year
Background: The Carbohydrate-Active enZymes (CAZy) auxiliary activity family 3 (AA3) comprises flavin adenine dinucleotide-dependent (FAD) oxidoreductases from the glucose–methanol–choline (GMC) family, which play auxiliary roles in lignocellulose conversion. The AA3 subfamily 1 predominantly consists of cellobiose dehydrogenases (CDHs) that typically comprise a dehydrogenase domain, a cytochrome domain, and a carbohydrate-binding module from family 1 (CBM1). Results: In this work, an AA3_1 gene from T. myriococcoides CBS 398.93 encoding only a GMC dehydrogenase domain was expressed in Aspergillus niger. Like previously characterized CDHs, this enzyme (TmXdhA) predominantly accepts linear saccharides with β-(1 → 4) linkage and targets the hydroxyl on the reducing anomeric carbon. TmXdhA was distinguished, however, by its preferential activity towards xylooligosaccharides over cellooligosaccharides. Amino acid sequence analysis showed that TmXdhA possesses a glutamine at the substrate-binding site rather than a threonine or serine that occupies this position in previously characterized CDHs, and structural models suggest the glutamine in TmXdhA could facilitate binding to pentose sugars. Conclusions: The biochemical analysis of TmXdhA revealed a catalytic preference for xylooligosaccharide substrates. The modeled structure of TmXdhA provides a reference for the screening of oxidoreductases targeting xylooligosaccharides. We anticipate TmXdhA to be a good candidate for the conversion of xylooligosaccharides to added-value chemicals by its exceptional catalytic ability.
|Number of pages||17|
|Journal||Biotechnology for Biofuels and Bioproducts|
|Publication status||Published - 7 Dec 2022|
|MoE publication type||A1 Journal article-refereed|
- CAZy AA3
- Cellobiose dehydrogenase
- Thermothelomyces myriococcoides
- Xylooligosaccharide dehydrogenase
FingerprintDive into the research topics of 'Characterization of a novel AA3_1 xylooligosaccharide dehydrogenase from Thermothelomyces myriococcoides CBS 398.93'. Together they form a unique fingerprint.
- 2 Finished
BIOSEMBL: Transaminases for BIOcatalytic cascades for hemicellulose reasSEMBLy
Master, E., Dahiya, D., Ioannou, E., Leamon, A. K. M. A. A. & Pohto, A.
31/10/2018 → 30/04/2022
Project: Other external funding: Other foreign funding
Capturing Oxidoreductases for Carbohydrate Oligomer Activation
01/09/2017 → 31/12/2020
Project: Academy of Finland: Other research funding