Characterization, genome analysis and genetic tractability studies of a new nanocellulose producing Komagataeibacter intermedius isolate

Pietro Cannazza; Antti J. Rissanen; Essi Sarlin; Dieval Guizelini; Carlotta Minardi; Pauli Losoi; Francesco Molinari; Diego Romano; Rahul Mangayil

doi:10.1038/s41598-022-24735-z

Characterization, genome analysis and genetic tractability studies of a new nanocellulose producing Komagataeibacter intermedius isolate

Pietro Cannazza
, Antti J. Rissanen
, Essi Sarlin
, Dieval Guizelini
, Carlotta Minardi
, Pauli Losoi
, Francesco Molinari
, Diego Romano
, Rahul Mangayil^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

12 Citations (Scopus)

135 Downloads (Pure)

Abstract

Bacterial nanocellulose (BC) is a highly versatile biopolymer currently pursued as a material of choice in varied themes of biomedical and material science research fields. With the aim to extend the biotechnological applications, the genetic tractability of the BC producers within the Komagataeibacter genus and its potential as an alternative host chassis in synthetic biology have been extensively studied. However, such studies have been largely focused on the model Komagataeibacter spp. Here, we present a novel K. intermedius strain capable of utilizing glucose, and glycerol sources for biomass and BC synthesis. Genome assembly identified one bacterial cellulose synthetase (bcs) operon containing the complete gene set encoding the BC biogenesis machinery (bcsI) and three additional copies (bcsII–IV). Investigations on the genetic tractability confirmed plasmid transformation, propagation of vectors with pBBR1 and p15A origin of replications and constitutive and inducible induction of recombinant protein in K. intermedius ENS15. This study provides the first report on the genetic tractability of K. intermedius, serving as starting point towards future genetic engineering of this strain.

Original language	English
Article number	20520
Number of pages	12
Journal	Scientific Reports
Volume	12
Issue number	1
DOIs	https://doi.org/10.1038/s41598-022-24735-z
Publication status	Published - 28 Nov 2022
MoE publication type	A1 Journal article-refereed

Funding

R.M., A.J.R., and D.R. thanks Academy of Finland (Project No’s. 323214 and 346983), Kone Foundation (Project No. 201803224) and Fondazione Cariplo (2020–1070), respectively, for supporting this study. R.M. would like to thank Mr. Rohith Krishnan for providing the Kombucha SCOBY. Open access funding provided by Tampere University, Finland. R.M., A.J.R., and D.R. thanks Academy of Finland (Project No’s. 323214 and 346983), Kone Foundation (Project No. 201803224) and Fondazione Cariplo (2020–1070), respectively, for supporting this study. R.M. would like to thank Mr. Rohith Krishnan for providing the Kombucha SCOBY. Open access funding provided by Tampere University, Finland.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 12 Responsible Consumption and Production

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Cite this

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title = "Characterization, genome analysis and genetic tractability studies of a new nanocellulose producing Komagataeibacter intermedius isolate",

abstract = "Bacterial nanocellulose (BC) is a highly versatile biopolymer currently pursued as a material of choice in varied themes of biomedical and material science research fields. With the aim to extend the biotechnological applications, the genetic tractability of the BC producers within the Komagataeibacter genus and its potential as an alternative host chassis in synthetic biology have been extensively studied. However, such studies have been largely focused on the model Komagataeibacter spp. Here, we present a novel K. intermedius strain capable of utilizing glucose, and glycerol sources for biomass and BC synthesis. Genome assembly identified one bacterial cellulose synthetase (bcs) operon containing the complete gene set encoding the BC biogenesis machinery (bcsI) and three additional copies (bcsII–IV). Investigations on the genetic tractability confirmed plasmid transformation, propagation of vectors with pBBR1 and p15A origin of replications and constitutive and inducible induction of recombinant protein in K. intermedius ENS15. This study provides the first report on the genetic tractability of K. intermedius, serving as starting point towards future genetic engineering of this strain.",

author = "Pietro Cannazza and Rissanen, \{Antti J.\} and Essi Sarlin and Dieval Guizelini and Carlotta Minardi and Pauli Losoi and Francesco Molinari and Diego Romano and Rahul Mangayil",

note = "Funding Information: R.M., A.J.R., and D.R. thanks Academy of Finland (Project No{\textquoteright}s. 323214 and 346983), Kone Foundation (Project No. 201803224) and Fondazione Cariplo (2020–1070), respectively, for supporting this study. R.M. would like to thank Mr. Rohith Krishnan for providing the Kombucha SCOBY. Open access funding provided by Tampere University, Finland.",

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AU - Cannazza, Pietro

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AU - Minardi, Carlotta

AU - Losoi, Pauli

AU - Molinari, Francesco

AU - Romano, Diego

AU - Mangayil, Rahul

N1 - Funding Information: R.M., A.J.R., and D.R. thanks Academy of Finland (Project No’s. 323214 and 346983), Kone Foundation (Project No. 201803224) and Fondazione Cariplo (2020–1070), respectively, for supporting this study. R.M. would like to thank Mr. Rohith Krishnan for providing the Kombucha SCOBY. Open access funding provided by Tampere University, Finland.

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N2 - Bacterial nanocellulose (BC) is a highly versatile biopolymer currently pursued as a material of choice in varied themes of biomedical and material science research fields. With the aim to extend the biotechnological applications, the genetic tractability of the BC producers within the Komagataeibacter genus and its potential as an alternative host chassis in synthetic biology have been extensively studied. However, such studies have been largely focused on the model Komagataeibacter spp. Here, we present a novel K. intermedius strain capable of utilizing glucose, and glycerol sources for biomass and BC synthesis. Genome assembly identified one bacterial cellulose synthetase (bcs) operon containing the complete gene set encoding the BC biogenesis machinery (bcsI) and three additional copies (bcsII–IV). Investigations on the genetic tractability confirmed plasmid transformation, propagation of vectors with pBBR1 and p15A origin of replications and constitutive and inducible induction of recombinant protein in K. intermedius ENS15. This study provides the first report on the genetic tractability of K. intermedius, serving as starting point towards future genetic engineering of this strain.

AB - Bacterial nanocellulose (BC) is a highly versatile biopolymer currently pursued as a material of choice in varied themes of biomedical and material science research fields. With the aim to extend the biotechnological applications, the genetic tractability of the BC producers within the Komagataeibacter genus and its potential as an alternative host chassis in synthetic biology have been extensively studied. However, such studies have been largely focused on the model Komagataeibacter spp. Here, we present a novel K. intermedius strain capable of utilizing glucose, and glycerol sources for biomass and BC synthesis. Genome assembly identified one bacterial cellulose synthetase (bcs) operon containing the complete gene set encoding the BC biogenesis machinery (bcsI) and three additional copies (bcsII–IV). Investigations on the genetic tractability confirmed plasmid transformation, propagation of vectors with pBBR1 and p15A origin of replications and constitutive and inducible induction of recombinant protein in K. intermedius ENS15. This study provides the first report on the genetic tractability of K. intermedius, serving as starting point towards future genetic engineering of this strain.

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Characterization, genome analysis and genetic tractability studies of a new nanocellulose producing Komagataeibacter intermedius isolate

Abstract

Funding

UN SDGs

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Other files and links

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ELM 4 PET/ Mangayil: Engineering protein secretion routes in Komagataeibacter rhaeticus to design growing functional materials

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Characterization, genome analysis and genetic tractability studies of a new nanocellulose producing Komagataeibacter intermedius isolate

Abstract

Funding

UN SDGs

Access to Document

Other files and links

Fingerprint

Projects

ELM 4 PET/ Mangayil: Engineering protein secretion routes in Komagataeibacter rhaeticus to design growing functional materials

Cite this