Cellular interactions of surface modified nanoporous silicon particles

Luis M. Bimbo*, Mirkka Sarparanta, Ermei Makila, Timo Laaksonen, Päivi Laaksonen, Jarno Salonen, Markus B. Linder, Jouni Hirvonen, Anu J. Airaksinen, Helder A. Santos

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

Abstract

In this study, the self-assembly of hydrophobin class II (HFBII) on the surface of thermally hydrocarbonized porous silicon (THCPSi) nanoparticles was investigated. The HFBII-coating converted the hydrophobic particles into more hydrophilic ones, improved the particles' cell viability in both HT-29 and Caco-2 cell lines compared to uncoated particles, and enhanced the particles' cellular association. The amount of HFBII adsorbed onto the particles was also successfully quantified by both the BCA assay and a HPLC method. Importantly, the permeation of a poorly water-soluble drug, indomethacin, loaded into THCPSi particles across Caco-2 monolayers was not affected by the protein coating. In addition, I-125-radiolabelled HFBII did not extensively permeate the Caco-2 monolayer and was found to be stably adsorbed onto the THCPSi nanoparticles incubated in pH 7.4, which renders the particles the possibility for further track-imaging applications. The results highlight the potential of HFBII coating for improving wettability, increasing biocompatibility and possible intestinal association of PSi nanoparticulates for drug delivery applications.

Original languageEnglish
Pages (from-to)3184-3192
Number of pages9
JournalNanoscale
Volume4
Issue number10
DOIs
Publication statusPublished - 2012
MoE publication typeA1 Journal article-refereed

Keywords

  • ORAL-DRUG DELIVERY
  • POROUS SILICON
  • MESOPOROUS SILICON
  • TRICHODERMA-REESEI
  • IN-VITRO
  • HYDROPHOBIN
  • MICROPARTICLES
  • NANOPARTICLES
  • BIOCOMPATIBILITY
  • PROTEIN

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