Cellular delivery of enzyme-loaded DNA origami

Ari Ora, Erika Järvihaavisto, Hongbo Zhang, Henni Auvinen, Hélder A. Santos, Mauri A. Kostiainen*, Veikko Linko

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

45 Citations (Scopus)
196 Downloads (Pure)

Abstract

In this communication, we show that active enzymes can be delivered into HEK293 cells in vitro when they are attached to tubular DNA origami nanostructures. We use bioluminescent enzymes as a cargo and monitor their activity from a cell lysate. The results show that the enzymes stay intact and retain their activity in the transfection process. The method is highly modular, which makes it a compelling candidate for a great variety of delivery applications.

Original languageEnglish
Pages (from-to)14161-14164
Number of pages4
JournalChemical Communications
Volume52
Issue number98
DOIs
Publication statusPublished - 2016
MoE publication typeA1 Journal article-refereed

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