Categorisation of sugar acid dehydratases in Aspergillus niger

Francine A. Motter; Joosu Kuivanen; Hanna Keränen; Satu Hilditch; Merja Penttilä; Peter Richard

doi:10.1016/j.fgb.2013.12.006

Categorisation of sugar acid dehydratases in Aspergillus niger

Francine A. Motter, Joosu Kuivanen, Hanna Keränen, Satu Hilditch, Merja Penttilä, Peter Richard^*

^*Corresponding author for this work

Not published at Aalto University

Research output: Contribution to journal › Article › Scientific › peer-review

13 Citations (Scopus)

Abstract

In the genome of Aspergillus niger five genes were identified coding for proteins with homologies to sugar acid dehydratases. The open reading frames were expressed in Saccharomyces cerevisiae and the activities tested with a library of sugar acids. Four genes were identified to code for proteins with activities with sugar acids: an L-galactonate dehydratase (gaaB), two D-galactonate dehydratases (dgdA, dgdB) and an L-rhamnonate dehydratase (lraC). The specificities of the proteins were characterised. The L-galactonate dehydratase had highest activity with L-fuconate, however it is unclear whether the enzyme is involved in L-fuconate catabolism. None of the proteins showed activity with galactaric acid or galactarolactone. (C) 2013 Elsevier Inc. All rights reserved.

Original language	English
Pages (from-to)	67-72
Number of pages	6
Journal	Fungal Genetics and Biology
Volume	64
DOIs	https://doi.org/10.1016/j.fgb.2013.12.006
Publication status	Published - Mar 2014
MoE publication type	A1 Journal article-refereed

Keywords

Dehydratase
2-Keto-3-deoxy acid
D-Galactonate
L-Galactonate
L-Rhamnonate
L-Fuconate
D-GALACTURONIC ACID
D-GALACTOSE
CATABOLIC PATHWAY
FILAMENTOUS FUNGI
ENZYMATIC-ACTIVITIES
ENOLASE SUPERFAMILY
MISSING LINK
PART
IDENTIFICATION
DEHYDROGENASE

Access to Document

10.1016/j.fgb.2013.12.006

Cite this

@article{f76f1eb913524c54a9f4249a185699cc,

title = "Categorisation of sugar acid dehydratases in Aspergillus niger",

abstract = "In the genome of Aspergillus niger five genes were identified coding for proteins with homologies to sugar acid dehydratases. The open reading frames were expressed in Saccharomyces cerevisiae and the activities tested with a library of sugar acids. Four genes were identified to code for proteins with activities with sugar acids: an L-galactonate dehydratase (gaaB), two D-galactonate dehydratases (dgdA, dgdB) and an L-rhamnonate dehydratase (lraC). The specificities of the proteins were characterised. The L-galactonate dehydratase had highest activity with L-fuconate, however it is unclear whether the enzyme is involved in L-fuconate catabolism. None of the proteins showed activity with galactaric acid or galactarolactone. (C) 2013 Elsevier Inc. All rights reserved.",

keywords = "Dehydratase, 2-Keto-3-deoxy acid, D-Galactonate, L-Galactonate, L-Rhamnonate, L-Fuconate, D-GALACTURONIC ACID, D-GALACTOSE, CATABOLIC PATHWAY, FILAMENTOUS FUNGI, ENZYMATIC-ACTIVITIES, ENOLASE SUPERFAMILY, MISSING LINK, PART, IDENTIFICATION, DEHYDROGENASE",

author = "Motter, {Francine A.} and Joosu Kuivanen and Hanna Ker{\"a}nen and Satu Hilditch and Merja Penttil{\"a} and Peter Richard",

year = "2014",

month = mar,

doi = "10.1016/j.fgb.2013.12.006",

language = "English",

volume = "64",

pages = "67--72",

journal = "Fungal Genetics and Biology",

issn = "1087-1845",

publisher = "Elsevier",

}

TY - JOUR

T1 - Categorisation of sugar acid dehydratases in Aspergillus niger

AU - Motter, Francine A.

AU - Kuivanen, Joosu

AU - Keränen, Hanna

AU - Hilditch, Satu

AU - Penttilä, Merja

AU - Richard, Peter

PY - 2014/3

Y1 - 2014/3

N2 - In the genome of Aspergillus niger five genes were identified coding for proteins with homologies to sugar acid dehydratases. The open reading frames were expressed in Saccharomyces cerevisiae and the activities tested with a library of sugar acids. Four genes were identified to code for proteins with activities with sugar acids: an L-galactonate dehydratase (gaaB), two D-galactonate dehydratases (dgdA, dgdB) and an L-rhamnonate dehydratase (lraC). The specificities of the proteins were characterised. The L-galactonate dehydratase had highest activity with L-fuconate, however it is unclear whether the enzyme is involved in L-fuconate catabolism. None of the proteins showed activity with galactaric acid or galactarolactone. (C) 2013 Elsevier Inc. All rights reserved.

AB - In the genome of Aspergillus niger five genes were identified coding for proteins with homologies to sugar acid dehydratases. The open reading frames were expressed in Saccharomyces cerevisiae and the activities tested with a library of sugar acids. Four genes were identified to code for proteins with activities with sugar acids: an L-galactonate dehydratase (gaaB), two D-galactonate dehydratases (dgdA, dgdB) and an L-rhamnonate dehydratase (lraC). The specificities of the proteins were characterised. The L-galactonate dehydratase had highest activity with L-fuconate, however it is unclear whether the enzyme is involved in L-fuconate catabolism. None of the proteins showed activity with galactaric acid or galactarolactone. (C) 2013 Elsevier Inc. All rights reserved.

KW - Dehydratase

KW - 2-Keto-3-deoxy acid

KW - D-Galactonate

KW - L-Galactonate

KW - L-Rhamnonate

KW - L-Fuconate

KW - D-GALACTURONIC ACID

KW - D-GALACTOSE

KW - CATABOLIC PATHWAY

KW - FILAMENTOUS FUNGI

KW - ENZYMATIC-ACTIVITIES

KW - ENOLASE SUPERFAMILY

KW - MISSING LINK

KW - PART

KW - IDENTIFICATION

KW - DEHYDROGENASE

U2 - 10.1016/j.fgb.2013.12.006

DO - 10.1016/j.fgb.2013.12.006

M3 - Article

SN - 1087-1845

VL - 64

SP - 67

EP - 72

JO - Fungal Genetics and Biology

JF - Fungal Genetics and Biology

ER -

Categorisation of sugar acid dehydratases in Aspergillus niger

Abstract

Keywords

Access to Document

Fingerprint

Cite this