Categorisation of sugar acid dehydratases in Aspergillus niger

Francine A. Motter, Joosu Kuivanen, Hanna Keränen, Satu Hilditch, Merja Penttilä, Peter Richard*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

9 Citations (Scopus)

Abstract

In the genome of Aspergillus niger five genes were identified coding for proteins with homologies to sugar acid dehydratases. The open reading frames were expressed in Saccharomyces cerevisiae and the activities tested with a library of sugar acids. Four genes were identified to code for proteins with activities with sugar acids: an L-galactonate dehydratase (gaaB), two D-galactonate dehydratases (dgdA, dgdB) and an L-rhamnonate dehydratase (lraC). The specificities of the proteins were characterised. The L-galactonate dehydratase had highest activity with L-fuconate, however it is unclear whether the enzyme is involved in L-fuconate catabolism. None of the proteins showed activity with galactaric acid or galactarolactone. (C) 2013 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)67-72
Number of pages6
JournalFungal Genetics and Biology
Volume64
DOIs
Publication statusPublished - Mar 2014
MoE publication typeA1 Journal article-refereed

Keywords

  • Dehydratase
  • 2-Keto-3-deoxy acid
  • D-Galactonate
  • L-Galactonate
  • L-Rhamnonate
  • L-Fuconate
  • D-GALACTURONIC ACID
  • D-GALACTOSE
  • CATABOLIC PATHWAY
  • FILAMENTOUS FUNGI
  • ENZYMATIC-ACTIVITIES
  • ENOLASE SUPERFAMILY
  • MISSING LINK
  • PART
  • IDENTIFICATION
  • DEHYDROGENASE

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