Catcher/Tag Toolbox: Biomolecular Click-Reactions For Protein Engineering Beyond Genetics

Ruxia Fan; A. Sesilja Aranko

doi:10.1002/cbic.202300600

Catcher/Tag Toolbox: Biomolecular Click-Reactions For Protein Engineering Beyond Genetics

Ruxia Fan, A. Sesilja Aranko^*

^*Corresponding author for this work

Research output: Contribution to journal › Review Article › peer-review

18 Citations (Scopus)

79 Downloads (Pure)

Abstract

Manipulating protein architectures beyond genetic control has attracted widespread attention. Catcher/Tag systems enable highly specific conjugation of proteins in vivo and in vitro via an isopeptide-bond. They provide efficient, robust, and irreversible strategies for protein conjugation and are simple yet powerful tools for a variety of applications in enzyme industry, vaccines, biomaterials, and cellular applications. Here we summarize recent development of the Catcher/Tag toolbox with a particular emphasis on the design of Catcher/Tag pairs targeted for specific applications. We cover the current limitations of the Catcher/Tag systems and discuss the pH sensitivity of the reactions. Finally, we conclude some of the future directions in the development of this versatile protein conjugation method and envision that improved control over inducing the ligation reaction will further broaden the range of applications.

Original language	English
Article number	e202300600
Number of pages	12
Journal	ChemBioChem
Volume	25
Issue number	1
Early online date	15 Nov 2023
DOIs	https://doi.org/10.1002/cbic.202300600
Publication status	Published - 2 Jan 2024
MoE publication type	A2 Review article, Literature review, Systematic review

Funding

. This work was supported by the Academy of Finland through its Centres of Excellence Programme Life‐Inspired Hybrid Materials (LIBER, 2022–2029) under project no. 346105 and Academy of Finland project no. 333238, and Novo Nordisk Foundation grant (NNF23OC0081564)

Keywords

Catcher/Tag system
isopeptide bond
protein conjugation
protein engineering
protein ligation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1002/cbic.202300600Licence: CC BY

CHEM_Fan_and_Aranko_CatcherTag_Toolbox_2024_ChemBioChemFinal published version, 2.49 MBLicence: CC BY

Cite this

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title = "Catcher/Tag Toolbox: Biomolecular Click-Reactions For Protein Engineering Beyond Genetics",

abstract = "Manipulating protein architectures beyond genetic control has attracted widespread attention. Catcher/Tag systems enable highly specific conjugation of proteins in vivo and in vitro via an isopeptide-bond. They provide efficient, robust, and irreversible strategies for protein conjugation and are simple yet powerful tools for a variety of applications in enzyme industry, vaccines, biomaterials, and cellular applications. Here we summarize recent development of the Catcher/Tag toolbox with a particular emphasis on the design of Catcher/Tag pairs targeted for specific applications. We cover the current limitations of the Catcher/Tag systems and discuss the pH sensitivity of the reactions. Finally, we conclude some of the future directions in the development of this versatile protein conjugation method and envision that improved control over inducing the ligation reaction will further broaden the range of applications.",

keywords = "Catcher/Tag system, isopeptide bond, protein conjugation, protein engineering, protein ligation",

author = "Ruxia Fan and Aranko, \{A. Sesilja\}",

note = "Funding Information: . This work was supported by the Academy of Finland through its Centres of Excellence Programme Life‐Inspired Hybrid Materials (LIBER, 2022–2029) under project no. 346105 and Academy of Finland project no. 333238, and Novo Nordisk Foundation grant (NNF23OC0081564) Publisher Copyright: {\textcopyright} 2023 The Authors. ChemBioChem published by Wiley-VCH GmbH.",

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AU - Fan, Ruxia

AU - Aranko, A. Sesilja

N1 - Funding Information: . This work was supported by the Academy of Finland through its Centres of Excellence Programme Life‐Inspired Hybrid Materials (LIBER, 2022–2029) under project no. 346105 and Academy of Finland project no. 333238, and Novo Nordisk Foundation grant (NNF23OC0081564) Publisher Copyright: © 2023 The Authors. ChemBioChem published by Wiley-VCH GmbH.

PY - 2024/1/2

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N2 - Manipulating protein architectures beyond genetic control has attracted widespread attention. Catcher/Tag systems enable highly specific conjugation of proteins in vivo and in vitro via an isopeptide-bond. They provide efficient, robust, and irreversible strategies for protein conjugation and are simple yet powerful tools for a variety of applications in enzyme industry, vaccines, biomaterials, and cellular applications. Here we summarize recent development of the Catcher/Tag toolbox with a particular emphasis on the design of Catcher/Tag pairs targeted for specific applications. We cover the current limitations of the Catcher/Tag systems and discuss the pH sensitivity of the reactions. Finally, we conclude some of the future directions in the development of this versatile protein conjugation method and envision that improved control over inducing the ligation reaction will further broaden the range of applications.

AB - Manipulating protein architectures beyond genetic control has attracted widespread attention. Catcher/Tag systems enable highly specific conjugation of proteins in vivo and in vitro via an isopeptide-bond. They provide efficient, robust, and irreversible strategies for protein conjugation and are simple yet powerful tools for a variety of applications in enzyme industry, vaccines, biomaterials, and cellular applications. Here we summarize recent development of the Catcher/Tag toolbox with a particular emphasis on the design of Catcher/Tag pairs targeted for specific applications. We cover the current limitations of the Catcher/Tag systems and discuss the pH sensitivity of the reactions. Finally, we conclude some of the future directions in the development of this versatile protein conjugation method and envision that improved control over inducing the ligation reaction will further broaden the range of applications.

KW - Catcher/Tag system

KW - isopeptide bond

KW - protein conjugation

KW - protein engineering

KW - protein ligation

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DO - 10.1002/cbic.202300600

M3 - Review Article

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AN - SCOPUS:85176389839

SN - 1439-4227

VL - 25

JO - ChemBioChem

JF - ChemBioChem

IS - 1

M1 - e202300600

ER -

Catcher/Tag Toolbox: Biomolecular Click-Reactions For Protein Engineering Beyond Genetics

Abstract

Funding

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

LIBER Linder: Life-like hybrid materials

CrossSilk: Durable and functional biomaterials from crosslinked non-canonical silk and cellulose composite

Cite this

Catcher/Tag Toolbox: Biomolecular Click-Reactions For Protein Engineering Beyond Genetics

Abstract

Funding

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Projects

LIBER Linder: Life-like hybrid materials

CrossSilk: Durable and functional biomaterials from crosslinked non-canonical silk and cellulose composite

Cite this