TY - JOUR
T1 - Carbon quantum dots in-situ relieve humic acids inhibition on methanogens while significantly increasing the abundance of Methanosarcinaceae
AU - Liu, Qiang
AU - Xu, Yuanting
AU - Li, Yeqing
AU - Ma, Chengjie
AU - Chen, Shuo
AU - Feng, Lu
AU - Xu, Quan
AU - Pan, Junting
AU - Peng, Bo
AU - Zhou, Hongjun
AU - Xu, Chunming
N1 - Funding Information:
The authors appreciate the National Natural Science Foundation of China ( 22278435 ), the Strategic Cooperation Technology Projects of CNPC and CUPB ( ZLZX2020-04 ), China Finland joint international funding by NSFC (No. 52211530034 ), the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences , Fundamental Research Funds for Central Non-profit Scientific Institution (No. 1610132020003 ).
Publisher Copyright:
© 2023
PY - 2023/9/20
Y1 - 2023/9/20
N2 - Monophenols form humic acids (HA) through polycondensation reaction in the anaerobic digestion (AD) process, which will inhibit AD process. Currently, metal ions are the option for in-situ relieving HA inhibition during AD, but excess metal ions are harmful to microorganisms. In this study, carbon quantum dots (CQDs, a non-metallic materials) were proposed to relieve HA inhibition in-situ. We investigated the effect of HA on AD acidification and methanation stage, and synthesized CQDs using sodium citrate (s-CQDs) and p-phenylenediamine (p-CQDs) as precursors to relieve the HA inhibition in-situ. Results showed that s-CQDs (3.0 g/L) significantly increased the cumulative CH4 yield from AD of ethanol with 1.0 g/L HA (1.9 times higher than that without s-CQDs). Microbiological analysis indicated the most dominant methanogen was Methanosarcinaceae, with richness of 89.7%. Compared to the HA inhibition system, the relative abundance of Methanosarcinaceae increased by 87.5%. The analysis of interaction mechanism between CQDs and HA indicated that s-CQDs has an in-situ binding effect to HA by reacting with -OH, C[dbnd]C, and -COOH. This study provided a novel means for in-situ relieving HA inhibition, and illustrated the interaction mechanism between CQDs and HA, which will guide the application in production of bioenergy.
AB - Monophenols form humic acids (HA) through polycondensation reaction in the anaerobic digestion (AD) process, which will inhibit AD process. Currently, metal ions are the option for in-situ relieving HA inhibition during AD, but excess metal ions are harmful to microorganisms. In this study, carbon quantum dots (CQDs, a non-metallic materials) were proposed to relieve HA inhibition in-situ. We investigated the effect of HA on AD acidification and methanation stage, and synthesized CQDs using sodium citrate (s-CQDs) and p-phenylenediamine (p-CQDs) as precursors to relieve the HA inhibition in-situ. Results showed that s-CQDs (3.0 g/L) significantly increased the cumulative CH4 yield from AD of ethanol with 1.0 g/L HA (1.9 times higher than that without s-CQDs). Microbiological analysis indicated the most dominant methanogen was Methanosarcinaceae, with richness of 89.7%. Compared to the HA inhibition system, the relative abundance of Methanosarcinaceae increased by 87.5%. The analysis of interaction mechanism between CQDs and HA indicated that s-CQDs has an in-situ binding effect to HA by reacting with -OH, C[dbnd]C, and -COOH. This study provided a novel means for in-situ relieving HA inhibition, and illustrated the interaction mechanism between CQDs and HA, which will guide the application in production of bioenergy.
KW - Anaerobic digestion
KW - Carbon quantum dots
KW - Humic acids
KW - Interaction
KW - Microbial community
UR - http://www.scopus.com/inward/record.url?scp=85166261996&partnerID=8YFLogxK
U2 - 10.1016/j.jclepro.2023.138258
DO - 10.1016/j.jclepro.2023.138258
M3 - Article
AN - SCOPUS:85166261996
SN - 0959-6526
VL - 419
JO - Journal of Cleaner Production
JF - Journal of Cleaner Production
M1 - 138258
ER -
